MicroRNA-21 is upregulated during intestinal barrier dysfunction induced by ischemia reperfusion.
|
| Abstract |
This study aimed to investigate the expression of miRNA-21 during intestinal barrier dysfunction induced by intestinal ischemia reperfusion. Forty SPF SD rats were divided into 5 groups randomly. Intestinal ischemia-reperfusion injury (IRI) was induced by mesenteric artery occlusion for 1 h and reperfusion for 1 h, and the rats were sacrificed at 1, 3, 6 and 12 h after reperfusion. Fresh intestine tissues were immediately isolated for the measurement of transepithelial electrical resistance (TER). The levels of cytokines, ICAM-1, DAO, iFABP and MPO in serum were determined by ELISA. Intestinal tight junction proteins occludin and claudin-1 were detected by immunofluorescence analysis and Western blot analysis. miR-21 expression in intestinal tissues was measured by RT-PCR. Compared with sham group, the levels of pro-inflammatory cytokines TNF-α and IL-6 and ICAM-1, DAO, iFABP and MPO increased while IL-10 level decreased in intestinal ischemia-reperfusion group. In addition, the levels of intestinal tight junction proteins occludin and claudin-1 decreased while miR-21 level increased in intestinal ischemia-reperfusion group, compared with sham group. In conclusion, miR-21 expression is upregulated during intestinal barrier dysfunction induced by IRI. miR-21 may play an important role in the regulation of intestinal barrier function.
|
|---|---|
| Authors | Lin Zhang, Feng Zhang, Dai-Kun He, Xiao-Ming Fan, Jie Shen |
| Journal | The Kaohsiung journal of medical sciences (Kaohsiung J Med Sci) Vol. 34 Issue 10 Pg. 556-563 (Oct 2018) ISSN: 2410-8650 [Electronic] China (Republic : 1949- ) |
| PMID | 30309483 (Publication Type: Journal Article) |
| Copyright | Copyright © 2018. Published by Elsevier Taiwan LLC. |
| Chemical References |
|
| Topics |
|
Join CureHunter, for free Research Interface BASIC access!
Realize the full power of the drug-disease research graph!