Performance of Hepatitis B Core-Related Antigen Versus Hepatitis B Surface Antigen and Hepatitis B Virus DNA in Predicting HBeAg-positive and HBeAg-negative Chronic Hepatitis.

Abstract

BACKGROUND:

We examined changes in hepatitis B core-related antigen (HBcrAg) during the four sequential phases of chronic hepatitis B virus (HBV) infection: hepatitis B e antigen (HBeAg)-positive chronic infection (EPCI) and hepatitis (EPCH), followed by HBeAg-negative chronic infection (ENCI) and hepatitis (ENCH). We compared the performance of serum HBcrAg, hepatitis B surface antigen (HBsAg), and HBV DNA in predicting EPCH and ENCH.

METHODS:

We enrolled 492 consecutive patients: 49 with EPCI, 243 with EPCH, 101 with ENCI, and 99 with ENCH. HBcrAg was detected by chemiluminescent enzyme immunoassays. HBsAg and HBeAg were detected by chemiluminescent microparticle immunoassays. HBV DNA was detected by real-time PCR. Predictive performance of HBcrAg, HBsAg, and HBV DNA was evaluated using ROC curves.

RESULTS:

Areas under ROC curves (AUCs) of HBcrAg, HBsAg, and HBV DNA for predicting EPCH were 0.738, 0.812, and 0.717, respectively; optimal cutoffs were ≤1.43×10⁵ kU/mL, ≤1.89×10⁴ IU/mL, and ≤3.97×10⁷ IU/mL, with sensitivities and specificities of 66.3% and 77.6%, 65.0% and 93.9%, and 60.5% and 79.6%, respectively. AUCs of HBcrAg, HBsAg, and HBV DNA for predicting ENCH were 0.887, 0.581, and 0.978, respectively; optimal cutoffs were >26.8 kU/mL, >2.29×10² IU/mL, and >8.75×10³ IU/mL, with sensitivities and specificities of 72.7% and 95.1%, 86.9% and 39.6%, and 89.9% and 92.1%, respectively.

CONCLUSIONS:

HBsAg and HBV DNA were the best predictors of EPCH and ENCH, respectively. HBcrAg is an important surrogate marker for predicting EPCH and ENCH.

• Authors: Zhan Qing Zhang, Yan Bing Wang, Wei Lu, Dan Ping Liu, Bi Sheng Shi, Xiao Nan Zhang, Dan Huang, Xiu Fen Li, Xin Lan Zhou, Rong Rong Ding
• Journal: Annals of laboratory medicine (Ann Lab Med) Vol. 39 Issue 1 Pg. 67-75 (Jan 2019) ISSN: 2234-3814 [Electronic] Korea (South)
• PMID: 30215232 (Publication Type: Journal Article)
• Copyright: © The Korean Society for Laboratory Medicine.

Chemical References

• DNA, Viral
• Hepatitis B Core Antigens
• Hepatitis B Surface Antigens
• Hepatitis B e Antigens

Topics

• Adolescent
• Adult
• Aged
• Area Under Curve
• DNA, Viral (blood)
• Female
• Hepatitis B Core Antigens (blood)
• Hepatitis B Surface Antigens (blood)
• Hepatitis B e Antigens (blood)
• Hepatitis B virus (genetics, metabolism)
• Hepatitis B, Chronic (diagnosis, virology)
• Humans
• Immunoassay
• Luminescent Measurements
• Male
• Middle Aged
• ROC Curve
• Real-Time Polymerase Chain Reaction
• Sensitivity and Specificity
• Young Adult