Antidiabetic activities of polysaccharides from Anoectochilus roxburghii and Anoectochilus formosanus in STZ-induced diabetic mice.

Abstract

Six polysaccharides were extracted from different parts (whole plants, roots and leaves) of Anoectochilus roxburghii and Anoectochilus formosanus (ARPs, ARPs-1, ARPs-2, AFPs, AFPs-1 and AFPs-2). Their primary characteristics were identified and their antidiabetic activities were evaluated in STZ-induced diabetic mice. Root polysaccharides (ARPs-1and AFPs-1) and leaf polysaccharides (ARPs-2 and AFPs-2) were distinct from each other in terms of the Mws, monosaccharide compositions and functional groups. Notably, the primary structures of ARPs and AFPs were similar to those of ARPs-1and AFPs-1, respectively. In animal experiment, after feeding with polysaccharides samples, the body weight, blood glucose, glycogen, insulin, total cholesterol (TC), triglyceride (TG), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), malondiadehyde (MDA) and antioxidant enzyme activities in liver and kidney of mice were tested to investigate the antidiabetic activities. All polysaccharides exhibited varying antidiabetic activities (antihyperglycemic, antioxidant and antihyperlipidemic activities), which were closely related to their primary characteristics. Furthermore, root polysaccharides with higher Mws and glucose content in both A. roxburghii and A. formosanus, exhibited better antidiabetic activities than leaf polysaccharides, and ARPs which showed the best antidiabetic activities had the potential to be used as functional food or medicine for diabetes treatment.

• Authors: Tingting Tang, Xiaoyu Duan, Yu Ke, Lan Zhang, Yingbin Shen, Bin Hu, Aiping Liu, Hong Chen, Cheng Li, Wenjuan Wu, Li Shen, Yuntao Liu
• Journal: International journal of biological macromolecules (Int J Biol Macromol) Vol. 112 Pg. 882-888 (Jun 2018) ISSN: 1879-0003 [Electronic] Netherlands
• PMID: 29438753 (Publication Type: Journal Article)
• Copyright: Copyright © 2018 Elsevier B.V. All rights reserved.

Chemical References

• Antioxidants
• Blood Glucose
• Hypoglycemic Agents
• Insulin
• Lipids
• Monosaccharides
• Polysaccharides
• Uronic Acids
• Malondialdehyde
• Streptozocin
• Glycogen

Topics

• Animals
• Antioxidants (metabolism)
• Blood Glucose (metabolism)
• Body Weight (drug effects)
• Diabetes Mellitus, Experimental (blood, drug therapy)
• Glycogen
• Hypoglycemic Agents (therapeutic use)
• Insulin (blood)
• Kidney (enzymology)
• Lipids (blood)
• Liver (enzymology)
• Male
• Malondialdehyde (metabolism)
• Mice, Inbred ICR
• Molecular Weight
• Monosaccharides (analysis)
• Orchidaceae (chemistry)
• Polysaccharides (pharmacology, therapeutic use)
• Spectroscopy, Fourier Transform Infrared
• Streptozocin
• Uronic Acids (analysis)