Effects of molsidomine on retinal ischemia/reperfusion injury in rabbits.

Abstract

We investigated the effect of molsidomine (MOL) on ischemia/reperfusion (I/R) injury. Rabbits were assigned to four groups: group 1, sham; group 2, I/R; group 3, MOL treatment for 4 days after I/R; group 4, MOL treatment for 1 day before I/R and 3 days after I/R. Retinal I/R was produced by elevating the intraocular pressure to 150 mm Hg for 60 min. Seven days after I/R, the eyes were enucleated. Retinal changes were examined using histochemistry. The levels of malondialdehyde (MDA), glutathione (GSH), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and catalase (CAT) also were measured. We found a significant increase in the thickness of the outer nuclear layer of group 3 compared to the other groups. In groups 3 and 4, caspase-3 stained cells in the ganglion cell layer were decreased compared to group 2. We found a significant increase in caspase-3 stained cells in the inner nuclear layer (INL) of group 2 compared to the other groups. We found a significant increase in caspase-3 stained cells in group 3 compared to group 4 in the INL. The MDA level in group 2 was significantly higher than group 1 and MOL significantly decreased MDA levels in groups 3 and 4. We found that MOL protected the retina from I/R injury by enhancing antioxidative effects and inhibiting apoptosis of retinal cells.

• Authors: N Polat, M A Ozer, H Parlakpinar, N Vardi, Z Aksungur, O Ozhan, A Yildiz, Y Turkoz
• Journal: Biotechnic & histochemistry : official publication of the Biological Stain Commission (Biotech Histochem) Vol. 93 Issue 3 Pg. 188-197 ( 2018) ISSN: 1473-7760 [Electronic] England
• PMID: 29323543 (Publication Type: Journal Article)

Chemical References

• Molsidomine

Topics

• Animals
• Immunohistochemistry
• Molsidomine (therapeutic use)
• Rabbits
• Rats
• Reference Standards
• Reperfusion Injury (drug therapy)
• Retina (drug effects)