Abstract | OBJECTIVE: METHODS: Female, 5-week-old IL-6 knockout (KO) and littermate mice were fed a normal diet (ND, 10% fat) or HFD (45% fat) for 14 weeks. At the end of treatment, cardiac function was assessed by echocardiography. Adipose tissues and plasma were collected for further measurement. Immunohistology of CD68 was performed to detect inflammation in the heart. Masson's trichrome staining and Oil Red O staining was applied to evaluated cardiac fibrosis and lipid accumulation. Real-time PCR and Western immunoblotting analyses on heart tissue were used to explore the underlying mechanism. RESULTS:
IL-6 KO mice displayed increased insulin resistance compared to WT mice at baseline. When fed HFD, IL-6 KO mice showed decreased gains in body weight and fat mass, increased insulin resistance relative to IL-6 KO mice feed ND. Furthermore, IL-6 KO mice developed cardiac dysfunction during HFD-induced obesity. Histological analysis suggested increased lipid accumulation, fibrosis and inflammation without affecting cardiac morphology during HFD treatment in the heart of IL-6 KO mice. Finally, IL-6 deficiency increased the phosphorylation of AMPK and ACC in the heart during HFD-induced obesity. CONCLUSION:
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Authors | Fan Chen, Dandan Chen, Xinmei Zhao, Shuai Yang, Zhe Li, Daniel Sanchis, Liang Jin, Xizhe Qiang, Kaiye Wang, Yitao Xu, Yubin Zhang, Junmei Ye |
Journal | Biochimica et biophysica acta. Molecular basis of disease
(Biochim Biophys Acta Mol Basis Dis)
Vol. 1863
Issue 12
Pg. 3128-3141
(12 2017)
ISSN: 0925-4439 [Print] Netherlands |
PMID | 28844956
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2017 Elsevier B.V. All rights reserved. |
Chemical References |
- Antigens, CD
- Antigens, Differentiation, Myelomonocytic
- CD68 antigen, human
- Fatty Acids
- Interleukin-6
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Topics |
- Adipose Tissue
(metabolism)
- Animals
- Antigens, CD
(metabolism)
- Antigens, Differentiation, Myelomonocytic
(metabolism)
- Cardiomegaly
(metabolism)
- Cardiomyopathies
(metabolism, physiopathology)
- Diet, High-Fat
- Fatty Acids
(metabolism)
- Female
- Fibrosis
(metabolism, physiopathology)
- Gene Knockout Techniques
- Heart
(physiopathology)
- Inflammation
(metabolism, pathology)
- Insulin Resistance
- Interleukin-6
(deficiency, genetics, metabolism)
- Lipid Metabolism
- Mice
- Mice, Knockout
- Myocardium
(metabolism, pathology)
- Obesity
(metabolism, physiopathology)
- Phosphorylation
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