Fenugreek (Trigonella foenum graecum), a medicinal herb with potent antihyperglycaemic and hypoglycaemic effects, is used to treat diabetes. This study is aimed to explore the interaction of
fenugreek seed extract (FSE) and HPT (hypothalamic-pituitary-thyroid) axis in context of
leptin secretion which have important role in normal and type-1 diabetic subjects. FSE (confirmed to contain
trigonelline,
diosgenin, 4
hydroxyisoleucine) was gavaged (0.25 gm/kg
body weight/day) to normal and
alloxan-induced type-1 diabetic rats for 4 weeks. Expression of hypothalamic
prepro-TRH (
Thyrotropin releasing hormone)
mRNA, serum levels of TRH,
TSH (Thyroid stimulating hormone), fT3, fT4,
insulin,
leptin,
glucose; thyroperoxidase activity and growth of thyroid gland, food intake, adiposity index were also studied FSE significantly down regulated
prepro-TRH mRNA expression; decreased serum TRH, TSH, fT3, fT4 levels, and regressed thyroid gland in FSE-fed normal and diabetic rats than those observed in normal diet-fed control and diabetic rats. FSE decreased (p<0.005-0.001) adiposity index and
leptin secretion, increased food intake and
body weight in all FSE-fed rats. FSE improved insulin secretion, decreased
glucose level but impaired HPT axis in diabetic rats, indicating
insulin-independent
central hypothyroidism. Results suggested that the dominant signal to hypothalamus suppressing HPT axis is the fall in
leptin level which i resulted from decreased adiposity index following FSE feeding. Fenugreek simultaneously having hypoglycaemic and hypothyroidal actions raises questions whether it can be safely used to treat diabetes and/or
hyperthyroidism as was suggested by many workers.