Dual-mode electrochemical analysis of microRNA-21 using gold nanoparticle-decorated MoS2 nanosheet.
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| Abstract |
The detection of microRNA plays an important role in early cancer diagnosis. Herein, a dual-mode electronic biosensor was developed for microRNA-21 (miRNA-21) detection based on gold nanoparticle-decorated MoS2 nanosheet (AuNPs@MoS2). A classical DNA "sandwich" structure was employed to construct MoS2-based electrochemical sensor, including capture DNA, target miRNA-21 and DNA-modified nanoprobe. [Fe(CN)6]3-/4- and [Ru(NH3)6]3+ were selected as electrochemical indicators to monitor the preparation process and evaluate the performance of MoS2-based electrochemical biosensor by electrochemical impedance spectroscopy (EIS) and differential pulse voltammetry (DPV), respectively. Such MoS2-based biosensor exhibited excellent performance for miRNA-21 detection in the range from 10 fM to 1nM with detection limit of 0.78fM and 0.45fM for DPV and EIS technique, respectively. Furthermore, the proposed MoS2-based biosensor displayed high selectivity and stability, which could be used to determine miRNA-21 in human serum samples with satisfactory results. All data suggested that such MoS2-based nanocomposite may be a potential candidate for biosensing ranging from nucleic acid to protein detection.
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| Authors | Shao Su, Wenfang Cao, Wei Liu, Zaiwei Lu, Dan Zhu, Jie Chao, Lixing Weng, Lihua Wang, Chunhai Fan, Lianhui Wang |
| Journal | Biosensors & bioelectronics (Biosens Bioelectron) Vol. 94 Pg. 552-559 (Aug 15 2017) ISSN: 1873-4235 [Electronic] England |
| PMID | 28363193 (Publication Type: Journal Article) |
| Copyright | Copyright © 2017 Elsevier B.V. All rights reserved. |
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