Abstract | BACKGROUND: METHODS: We conducted a prospective experimental interventional study on wild-type (WT) and PTX3-deficient (PTX3-/-) mice. Acute lung injury was induced in WT and PTX3-/- mice by instillation of hydrochloric acid into the right bronchus. One hour later, animals received intraperitoneal sterile phosphate-buffered saline (PBS), WT-MSCs (1 × 106) or PTX3-/--MSCs (1 × 106). Twenty-four hours after injury, we measured the effects of treatments on arterial blood gases, wet/dry lung weight (W/D), CT scan analysis of lung collapse, neutrophils, TNFα and CXCL1 in bronchoalveolar lavage, and plasma PTX3. D-dimer was assayed in 1 week and OH- proline in 2 weeks to track the fibrotic evolution. RESULTS: In 24 h, in comparison to PBS, WT-MSCs improved oxygenation and reduced W/D and alveolar collapse. These effects were associated with decreased concentrations of alveolar neutrophils and cytokines. WT-MSCs increased D-dimer concentration and decreased OH- proline levels, too. Treatment with PTX3-/--MSCs ameliorated oxygenation, W/D, and alveolar TNFα, though to a lesser extent than WT-MSCs. PTX3-/--MSCs did not improve lung collapse, neutrophil count, CXCL1, D-dimer, and OH- proline concentrations. The protective effects of WT-MSCs were dampened by lack of endogenous PTX3, too. CONCLUSIONS:
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Authors | Tommaso Mauri, Vanessa Zambelli, Claudia Cappuzzello, Giacomo Bellani, Erica Dander, Marina Sironi, Vittoria Castiglioni, Andrea Doni, Alberto Mantovani, Andrea Biondi, Cecilia Garlanda, Giovanna D'amico, Antonio Pesenti |
Journal | Intensive care medicine experimental
(Intensive Care Med Exp)
Vol. 5
Issue 1
Pg. 13
(Dec 2017)
ISSN: 2197-425X [Print] Germany |
PMID | 28265979
(Publication Type: Journal Article)
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