Therapeutic Effects of Human Umbilical Cord-Derived Mesenchymal Stem Cells in Acute Lung Injury Mice.

Abstract

The incidence and mortality of acute lung injury (ALI)/acute respiratory distress syndrome (ARDS) are still very high, but stem cells show some promise for its treatment. Here we found that intratracheal administration of human umbilical cord-mesenchymal stem cells (UC-MSCs) significantly improved survival and attenuated the lung inflammation in lipopolysaccharide (LPS)-induced ALI mice. We also used the proteins-chip and bioinformatics to analyze interactions between UC-MSCs treatment and immune-response alternations of ALI mice. Then we demonstrated that UC-MSCs could inhibit the inflammatory response of mouse macrophage in ALI mice, as well as enhance its IL-10 expression. We provide data to support the concept that the therapeutic capacity of UC-MSCs for ALI was primarily through paracrine secretion, particularly of prostaglandin-E2 (PGE2). Furthermore, we showed that UC-MSCs might secrete a panel of factors including GM-CSF, IL-6 and IL-13 to ameliorate ALI. Our study suggested that UC-MSCs could protect LPS-induced ALI model by immune regulation and paracrine factors, indicating that UC-MSCs should be a promising strategy for ALI/ARDS.

• Authors: Hua Zhu, Yi Xiong, Yunqiu Xia, Rong Zhang, Daiyin Tian, Ting Wang, Jihong Dai, Lijia Wang, Hongbing Yao, Hong Jiang, Ke Yang, Enmei Liu, Yujun Shi, Zhou Fu, Li Gao, Lin Zou
• Journal: Scientific reports (Sci Rep) Vol. 7 Pg. 39889 (01 04 2017) ISSN: 2045-2322 [Electronic] England
• PMID: 28051154 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Lipopolysaccharides
• Interleukin-10
• Granulocyte-Macrophage Colony-Stimulating Factor
• Dinoprostone

Topics

• Acute Lung Injury (etiology, mortality, therapy)
• Animals
• Bronchoalveolar Lavage Fluid (chemistry)
• Cells, Cultured
• Dinoprostone (metabolism)
• Disease Models, Animal
• Female
• Granulocyte-Macrophage Colony-Stimulating Factor (metabolism)
• Humans
• Interleukin-10 (metabolism)
• Lipopolysaccharides (toxicity)
• Lung (metabolism, pathology)
• Macrophages (cytology, metabolism)
• Mesenchymal Stem Cell Transplantation
• Mesenchymal Stem Cells (cytology, metabolism)
• Mice
• Mice, Inbred BALB C
• Mice, Inbred C57BL
• Survival Rate
• Umbilical Cord (cytology)