Abstract | BACKGROUND: PURPOSE: DESIGN/METHODS: Sleep-wake behavior of the SHRs was detected by electroencephalography (EEG) and electromyography (EMG) recordings. Blood pressure was measured by noninvasive blood pressure tail cuff test. Immunohistochemistry was performed to evaluate the noradrenergic neuronal activity. The level of norepinephrine (NE) was detected by HPLC-ECD. RESULTS:
Amlodipine (100mg/kg, i.g.), the well-known L-type Ca2+ channel blockers (CCBs) exhibited remarkable antihypertensive activities in SHRs, but did not show effects on sleep of SHRs. Tetrandrine (30 and 60mg/kg/day, i.g.) significantly suppressed blood pressure of SHRs. Meanwhile, tetrandrine (60mg/kg/day, i.g.) remarkably increased non-rapid eye movement sleep (NREMS) time, bouts and mean duration. The hypnotic effect of tetrandrine was potentiated by prazosin (0.5mg/kg, i.p.) but attenuated by yohimbine (2mg/kg, i.p.). Administration of tetrandrine (60mg/kg/day, i.g.) not only significantly decreased c-Fos positive ratio of noradrenergic neurons in the locus coeruleus (LC), but also significantly decrease NE in the endogenous sleep-wake regulating pathways including LC, hypothalamus and ventrolateral preoptic nucleus (VLPO). CONCLUSION:
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Authors | Yuan-Li Huang, Su-Ying Cui, Xiang-Yu Cui, Qing Cao, Hui Ding, Jin-Zhi Song, Xiao Hu, Hui Ye, Bin Yu, Zhao-Fu Sheng, Zi-Jun Wang, Yong-He Zhang |
Journal | Phytomedicine : international journal of phytotherapy and phytopharmacology
(Phytomedicine)
Vol. 23
Issue 14
Pg. 1821-1829
(Dec 15 2016)
ISSN: 1618-095X [Electronic] Germany |
PMID | 27912885
(Publication Type: Journal Article)
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Copyright | Copyright © 2016 Elsevier GmbH. All rights reserved. |
Chemical References |
- Alkaloids
- Antihypertensive Agents
- Benzylisoquinolines
- Calcium Channels, L-Type
- Hypnotics and Sedatives
- Plant Extracts
- tetrandrine
- Norepinephrine
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Topics |
- Alkaloids
(pharmacology, therapeutic use)
- Animals
- Antihypertensive Agents
(pharmacology, therapeutic use)
- Benzylisoquinolines
(pharmacology, therapeutic use)
- Blood Pressure
(drug effects)
- Calcium Channels, L-Type
(metabolism)
- Cross-Sectional Studies
- Electroencephalography
- Hypertension
(drug therapy, metabolism, physiopathology)
- Hypnotics and Sedatives
(pharmacology, therapeutic use)
- Male
- Norepinephrine
(metabolism)
- Phytotherapy
- Plant Extracts
(pharmacology, therapeutic use)
- Rats, Inbred SHR
- Sleep
(drug effects)
- Stephania tetrandra
(chemistry)
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