A silk peptide fraction restores cognitive function in AF64A-induced Alzheimer disease model rats by increasing expression of choline acetyltransferase gene.

Abstract

This study investigated the effects of a silk peptide fraction obtained by incubating silk proteins with Protease N and Neutrase (SP-NN) on cognitive dysfunction of Alzheimer disease model rats. In order to elucidate underlying mechanisms, the effect of SP-NN on the expression of choline acetyltransferase (ChAT) mRNA was assessed in F3.ChAT neural stem cells and Neuro2a neuroblastoma cells; active amino acid sequence was identified using HPLC-MS. The expression of ChAT mRNA in F3.ChAT cells increased by 3.79-fold of the control level by treatment with SP-NN fraction. The active peptide in SP-NN was identified as tyrosine-glycine with 238.1 of molecular weight. Male rats were orally administered with SP-NN (50 or 300mg/kg) and challenged with a cholinotoxin AF64A. As a result of brain injury and decreased brain acetylcholine level, AF64A induced astrocytic activation, resulting in impairment of learning and memory function. Treatment with SP-NN exerted recovering activities on acetylcholine depletion and brain injury, as well as cognitive deficit induced by AF64A. The results indicate that, in addition to a neuroprotective activity, the SP-NN preparation restores cognitive function of Alzheimer disease model rats by increasing the release of acetylcholine.

• Authors: Yeseul Cha, Sang Hoon Lee, Su Kil Jang, Haiyu Guo, Young-Hwan Ban, Dongsun Park, Gwi Yeong Jang, Sungho Yeon, Jeong-Yong Lee, Ehn-Kyoung Choi, Seong Soo Joo, Heon-Sang Jeong, Yun-Bae Kim
• Journal: Toxicology and applied pharmacology (Toxicol Appl Pharmacol) Vol. 314 Pg. 48-54 (Jan 01 2017) ISSN: 1096-0333 [Electronic] United States
• PMID: 27871887 (Publication Type: Journal Article)
• Copyright: Copyright © 2016 Elsevier Inc. All rights reserved.

Chemical References

• Aziridines
• Insect Proteins
• Peptide Fragments
• Silk
• ethylcholine aziridinium
• Choline O-Acetyltransferase
• Choline

Topics

• Alzheimer Disease (chemically induced, psychology)
• Animals
• Avoidance Learning (drug effects)
• Aziridines (toxicity)
• Cell Line, Tumor
• Choline (analogs & derivatives, toxicity)
• Choline O-Acetyltransferase (genetics)
• Cognition (drug effects)
• Disease Models, Animal
• Gene Expression Regulation, Enzymologic
• Insect Proteins (chemistry)
• Male
• Maze Learning (drug effects)
• Peptide Fragments (pharmacology)
• Rats
• Rats, Sprague-Dawley
• Silk (chemistry)