Serum Decorin, Interleukin-1β, and Transforming Growth Factor-β Predict Hypertrophic Scarring Postburn.

Abstract

Hypertrophic scar after burn injury is a significant problem. Previous studies have examined the roles for decorin, interleukin-1β, and transforming growth factor-β1 in hypertrophic scar formation locally, but few have considered their systemic influence. The authors conducted a pilot study to examine whether serum levels of these molecules could predict hypertrophic scar formation. Serum levels were measured using enzyme-linked immunosorbent assay, and hypertrophic scar formation determined from chart reviews. Peripheral blood mononuclear cells and fibroblasts were stimulated with decorin, interleukin-1β, and transforming growth factor-β1, and expression of profibrotic molecules examined using flow cytometry, immunofluorescence microscopy, quantitative polymerase chain reaction, and mass spectrometry. Multiple linear regression analysis suggested early serum levels of decorin and interleukin-1β, and late serum levels of transforming growth factor-β1 were predictive of hypertrophic scar formation. Decorin up-regulated the expression of toll-like receptor 4 and C-X-C receptor 4 in peripheral blood mononuclear cells, and interleukin-1β up-regulated fibroblast production of C-X-C ligand 12. Transforming growth factor-β1 up-regulated, and interleukin-1β down-regulated, the production of profibrotic cytokines, collagen, and myofibroblast differentiation. The model predicting hypertrophic scar formation is supported by clinical results and limited in vitro experiments.

• Authors: Peter O Kwan, Jie Ding, Edward E Tredget
• Journal: Journal of burn care & research : official publication of the American Burn Association (J Burn Care Res) 2016 Nov/Dec Vol. 37 Issue 6 Pg. 356-366 ISSN: 1559-0488 [Electronic] England
• PMID: 27828834 (Publication Type: Journal Article)

Chemical References

• CXCL12 protein, human
• CXCR4 protein, human
• Chemokine CXCL12
• Decorin
• IL1B protein, human
• Interleukin-1beta
• Receptors, CXCR4
• TLR4 protein, human
• Toll-Like Receptor 4
• Transforming Growth Factor beta

Topics

• Adult
• Burns (blood, pathology)
• Cells, Cultured
• Chemokine CXCL12 (metabolism)
• Cicatrix, Hypertrophic (blood, diagnosis)
• Decorin (blood)
• Female
• Fibroblasts (cytology)
• Humans
• Interleukin-1beta (blood)
• Leukocytes, Mononuclear (cytology)
• Male
• Middle Aged
• Pilot Projects
• Receptors, CXCR4 (metabolism)
• Toll-Like Receptor 4 (metabolism)
• Transforming Growth Factor beta (blood)
• Young Adult