Abstract | OBJECTIVES: METHODS: Rats were used for this study and an ANP model was induced by injecting 5% sodium taurocholate into the biliary-pancreatic duct. Experiments were performed in 3 groups: sham, ANP, and ANP + HRS (HRS). Animals were killed at 3, 12, and 24 hours after operation, and then blood and tissue samples were harvested. Various physiological, histological, and cellular and molecular parameters were analyzed. RESULTS: Analyses of serum, lipase, alanine transaminase, and aspartate aminotransferase indicated that ANP-induced AHI model was established successfully and HRS attenuated hepatic dysfunction. Hepatic superoxide dismutase and malondialdehyde levels showed HRS against oxidative stress. Cellular and molecular analyses including p-p38, p-JNK, p-ERK, and caspase-3, caspase-9, NF-κB, and TNF-α in hepatic tissues revealed that HRS attenuated ANP-induced AHI by inhibiting apoptosis and phosphorylation of JNK and p38, as well as NF-κB activation. CONCLUSIONS:
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Authors | Qiao Shi, Chen Chen, Wen-Hong Deng, Peng Wang, Teng Zuo, Liang Zhao, Jia Yu, Kai-Liang Zhao, Fang-Chao Mei, Chen Li, Gui-Rong Wang, Wei-Xing Wang |
Journal | Pancreas
(Pancreas)
Vol. 45
Issue 10
Pg. 1424-1431
(11 2016)
ISSN: 1536-4828 [Electronic] United States |
PMID | 27518466
(Publication Type: Journal Article)
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Chemical References |
- Reactive Oxygen Species
- Hydrogen
- p38 Mitogen-Activated Protein Kinases
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Topics |
- Animals
- Apoptosis
- Hydrogen
- Inflammation
- Pancreatitis, Acute Necrotizing
- Rats
- Reactive Oxygen Species
- p38 Mitogen-Activated Protein Kinases
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