Microdialysis and proteomics of subcutaneous interstitial fluid reveals increased galectin-1 in type 2 diabetes patients.

Abstract

OBJECTIVE:

To identify a potential therapeutic target for type 2 diabetes by comparing the subcutaneous interstitial fluid from type 2 diabetes patients and healthy men.

METHODS:

Proteomics was performed on the interstitial fluid of subcutaneous adipose tissue obtained by microdialysis from 7 type 2 diabetes patients and 8 healthy participants. 851 proteins were detected, of which 36 (including galectin-1) showed significantly altered expression in type 2 diabetes. We also measured galectin-1 expression in: (1) adipocytes isolated from adipose tissue biopsies from these participants; (2) subcutaneous adipose tissue of 24 obese participants before, during and after 16weeks on a very low calorie diet (VLCD); and (3) adipocytes isolated from 6 healthy young participants after 4weeks on a diet and lifestyle intervention to promote weight gain. We also determined the effect of galectin-1 on glucose uptake in human adipose tissue.

RESULTS:

Galectin-1 protein levels were elevated in subcutaneous dialysates from type 2 diabetes compared with healthy controls (p<0.05). In agreement, galectin-1 mRNA expression was increased in adipocytes from the type 2 diabetes patients (p<0.05). Furthermore, galectin-1 mRNA expression was decreased in adipose tissue after VLCD (p<0.05) and increased by overfeeding (p<0.05). Co-incubation of isolated human adipocytes with galectin-1 reduced glucose uptake (p<0.05) but this was independent of the insulin signal.

CONCLUSION:

Proteomics of the interstitial fluid in subcutaneous adipose tissue in vivo identified a novel adipokine, galectin-1, with a potential role in the pathophysiology of type 2 diabetes.

• Authors: Emanuel Fryk, Jeanna Perman Sundelin, Lena Strindberg, Maria J Pereira, Massimo Federici, Nikolaus Marx, Fredrik H Nyström, Martin Schmelz, Per-Arne Svensson, Jan W Eriksson, Jan Borén, Per-Anders Jansson
• Journal: Metabolism: clinical and experimental (Metabolism) Vol. 65 Issue 7 Pg. 998-1006 (07 2016) ISSN: 1532-8600 [Electronic] United States
• PMID: 27282870 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2016 Elsevier Inc. All rights reserved.

Chemical References

• Galectin 1

Topics

• Adult
• Aged
• Diabetes Mellitus, Type 2 (metabolism)
• Extracellular Fluid (chemistry)
• Galectin 1 (analysis)
• Humans
• Male
• Middle Aged
• Proteomics