Abstract | AIMS: MAIN METHODS: AF rat model was established using acetylcholine (ACh)-CaCl2 injection for 7days followed by ACh infusion into the heart. Prior to ACh infusion, ranolazine at 10.7mg/kg/0.5ml was injected into vein and followed by 0.56mg/kg/min infusion. Blood pressure and electrocardiogram were monitored during the infusion. Histological changes of atrial tissue were observed after H&E staining. Activities and protein expression of NADPH oxidase-4, xanthine oxidase, glutathione peroxidase and superoxide dismutase were examined using commercial assay kits and Western botting, respectively. Mitochondrial functions were evaluated through membrane potential, ATP production, activities of complex I and III and reactive oxygen species production. Apoptosis was measured using TUNEL staining. Protein expression of apoptotic proteins Bcl-2, Bax and cleaved- caspase 3 and Akt/mTOR signaling proteins were detected using Western blotting. KEY FINDINGS: Results demonstrated that ranolazine attenuated AF in ACh-CaCl2-exposed rats. In addition, ranolazine restored mitochondrial function, suppressed oxidative stress, and inhibited atrial cells apoptosis. Furthermore, the activated Akt/mTOR signaling pathway induced by AF was further activated by ranolazine. SIGNIFICANCE: The present study confirms the effects of ranolazine on AF rats induced by ACh-CaCl2, and provides evidence that the anti-AF effects are associated with the restoration of mitochondrial function and activation of the Akt/mTOR signaling pathway in atrial tissue.
|
Authors | Deling Zou, Ning Geng, Yanli Chen, Li Ren, Xingli Liu, Jiye Wan, Sicong Guo, Shaojun Wang |
Journal | Life sciences
(Life Sci)
Vol. 156
Pg. 7-14
(Jul 01 2016)
ISSN: 1879-0631 [Electronic] Netherlands |
PMID | 27208652
(Publication Type: Journal Article)
|
Copyright | Copyright © 2016 Elsevier Inc. All rights reserved. |
Chemical References |
- Ranolazine
- Proto-Oncogene Proteins c-akt
- TOR Serine-Threonine Kinases
- Calcium Chloride
- Acetylcholine
|
Topics |
- Acetylcholine
- Animals
- Apoptosis
(drug effects)
- Atrial Fibrillation
(pathology)
- Calcium Chloride
- Heart Atria
(drug effects, metabolism, pathology)
- Hemodynamics
(drug effects)
- Male
- Mitochondria
(drug effects, metabolism)
- Oxidative Stress
(drug effects)
- Proto-Oncogene Proteins c-akt
(metabolism)
- Ranolazine
(pharmacology)
- Rats, Sprague-Dawley
- Signal Transduction
(drug effects)
- TOR Serine-Threonine Kinases
(metabolism)
|