Young Barley Indicates Antitumor Effects in Experimental Breast Cancer In Vivo and In Vitro.

Abstract

The effect of dietary administered young barley containing a mixture of phytochemicals to female rats for the prevention of N-methyl-N-nitrosourea-induced mammary carcinogenesis was evaluated. After carcinogen administration (14 wk), mammary tumors were removed and prepared for histopathological and immunohistochemical analysis. Moreover, in vitro evaluation of possible mechanisms in MCF-7 breast cancer cell line was performed. Barley (0.3%) demonstrated mild antitumor effect in mammary carcinogenesis, yet 3% barley did not further improve this effect. Immunohistochemical analysis of rat tumor cells in treated groups showed significant increase in caspase-3 expression and significant reduction in Ki67 expression. In addition, 3% barley significantly decreased dityrosine levels versus control. Barley in higher dose significantly decreased serum low-density lipoprotein-cholesterol in rats. In vitro studies showed that barley significantly decreased survival of MCF-7 cells in 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay and significantly decreased 5-bromo-20-deoxyuridine incorporation versus control. Barley prevented cell cycle progression and extended incubation with barley showed significant increase in the percentage of annexin V/propidium iodide-positive MCF-7 cells. Our results propose an antitumor effect for the mixture of phytochemicals present in young barley in a breast cancer model.

• Authors: Peter Kubatka, Martin Kello, Karol Kajo, Peter Kruzliak, Desanka Výbohová, Karel Šmejkal, Petr Maršík, Anthony Zulli, Gabriela Gönciová, Ján Mojžiš, Andrea Kapinová, Radovan Murin, Martin Péč, Marián Adamkov, Ronald M Przygodzki
• Journal: Nutrition and cancer (Nutr Cancer) 2016 May-Jun Vol. 68 Issue 4 Pg. 611-21 ISSN: 1532-7914 [Electronic] United States
• PMID: 27042893 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Anticarcinogenic Agents
• Flavonoids
• Methylnitrosourea

Topics

• Animals
• Anticarcinogenic Agents (pharmacology)
• Apoptosis
• Breast Neoplasms
• Cell Proliferation
• Female
• Flavonoids (analysis)
• Hordeum (chemistry)
• Humans
• Lipid Metabolism
• MCF-7 Cells
• Mammary Neoplasms, Experimental (chemically induced, pathology, prevention & control)
• Methylnitrosourea (toxicity)
• Rats, Sprague-Dawley