Monitoring of an antigen manufacturing process.

Abstract

Fluorescence spectroscopy in combination with multivariate statistical methods was employed as a tool for monitoring the manufacturing process of pertactin (PRN), one of the virulence factors of Bordetella pertussis utilized in whopping cough vaccines. Fluorophores such as amino acids and co-enzymes were detected throughout the process. The fluorescence data collected at different stages of the fermentation and purification process were treated employing principal component analysis (PCA). Through PCA, it was feasible to identify sources of variability in PRN production. Then, partial least square (PLS) was employed to correlate the fluorescence spectra obtained from pure PRN samples and the final protein content measured by a Kjeldahl test from these samples. In view that a statistically significant correlation was found between fluorescence and PRN levels, this approach could be further used as a method to predict the final protein content.

• Authors: Vanessa Zavatti, Hector Budman, Raymond Legge, Melih Tamer
• Journal: Bioprocess and biosystems engineering (Bioprocess Biosyst Eng) Vol. 39 Issue 6 Pg. 855-69 (Jun 2016) ISSN: 1615-7605 [Electronic] Germany
• PMID: 26879644 (Publication Type: Journal Article)

Chemical References

• Antigens, Bacterial

Topics

• Antigens, Bacterial (chemistry)
• Bordetella pertussis (immunology)
• Principal Component Analysis
• Spectrometry, Fluorescence