5-Aminolevulinic acid combined with ferrous iron ameliorate ischemia-reperfusion injury in the mouse fatty liver model.

Abstract

BACKGROUND:

The fatty liver could increase the risk of serious acute ischemia reperfusion (I/R) injury, and hepatic steatosis is indeed a major risk factor for hepatic failure after grafting a fatty liver.

MATERIALS & METHODS:

Fatty liver models of methionine- and choline-deficient high-fat mice were subjected to I/R injury with or without 5-aminolevulinic acid (5-ALA)/sodium ferrous citrate (SFC) treatment. Levels of hepatic enzymes, lipid peroxidation and apoptosis, inflammatory cytokines and heme oxygenase (HO)-1, and the carbon monoxide (CO) in the liver, and reactive oxygen species (ROS), inflammatory cytokines and members of the signaling pathway in isolated Kupffer were assessed.

RESULTS:

Alanine aminotransferase and aspartate aminotransferase levels, the number of necrotic areas, thiobarbituric acid reactive substance content, TUNEL-positive cells, infiltrated macrophages, and the inflammatory cytokine expression after I/R injury were dramatically decreased, whereas the endogenous CO concentrations and the HO-1 expression were significantly increased by 5-ALA/SFC treatment. The expression of toll-like receptors 2 and 4, NF-κB and inflammatory cytokines and ROS production in Kupffer cells were significantly decreased with 5-ALA/SFC treatment.

CONCLUSION:

5-ALA/SFC significantly attenuates the injury level in the fatty liver after I/R injury.

• Authors: Shaowei Li, Terumi Takahara, Xiao-Kang Li, Masayuki Fujino, Toshiro Sugiyama, Kazuhiro Tsukada, Chi Liu, Yoichi Kakuta, Norio Nonomura, Hidenori Ito, Kiwamu Takahashi, Motowo Nakajima, Tohru Tanaka, Shiro Takahara
• Journal: Biochemical and biophysical research communications (Biochem Biophys Res Commun) Vol. 470 Issue 4 Pg. 900-6 (Feb 19 2016) ISSN: 1090-2104 [Electronic] United States
• PMID: 26820535 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2016 Elsevier Inc. All rights reserved.

Chemical References

• Cytokines
• Drug Combinations
• Ferrous Compounds
• Reactive Oxygen Species
• ferrous sulfate
• Aminolevulinic Acid

Topics

• Aminolevulinic Acid (administration & dosage)
• Animals
• Cytokines (immunology)
• Drug Combinations
• Fatty Liver (drug therapy, immunology, pathology)
• Ferrous Compounds (administration & dosage)
• Kupffer Cells (drug effects, immunology)
• Mice
• Reactive Oxygen Species (immunology)
• Reperfusion Injury (drug therapy, immunology, pathology)
• Treatment Outcome