Abstract |
Coxsackievirus A16 (CA16) is a dominant pathogen that results in hand, foot, and mouth disease and causes outbreaks worldwide, particularly in the Asia-Pacific region. However, the underlying molecular mechanisms remain unclear. Our previous study has demonstrated that the basic CA16 pathogenic process was successfully mimicked in rhesus monkey infant. The present study focused on the global gene expression changes in peripheral blood mononuclear cells of rhesus monkey infants with hand, foot, and mouth disease induced by CA16 infection at different time points. Genome-wide expression analysis was performed with Agilent whole-genome microarrays and established bioinformatics tools. Nine hundred and forty-eight significant differentially expressed genes that were associated with 5 gene ontology categories, including cell communication, cell cycle, immune system process, regulation of transcription and metabolic process were identified. Subsequently, the mapping of genes related to the immune system process by PANTHER pathway analysis revealed the predominance of inflammation mediated by chemokine and cytokine signaling pathways and the interleukin signaling pathway. Ultimately, co-expressed genes and their networks were analyzed. The results revealed the gene expression profile of the immune system in response to CA16 in rhesus monkey infants and suggested that such an immune response was generated as a result of the positive mobilization of the immune system. This initial microarray study will provide insights into the molecular mechanism of CA16 infection and will facilitate the identification of biomarkers for the evaluation of vaccines against this virus.
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Authors | Jie Song, Yajie Hu, Yunguang Hu, Jingjing Wang, Xiaolong Zhang, Lichun Wang, Lei Guo, Yancui Wang, Ruotong Ning, Yun Liao, Ying Zhang, Huiwen Zheng, Haijing Shi, Zhanlong He, Qihan Li, Longding Liu |
Journal | Virus research
(Virus Res)
Vol. 214
Pg. 1-10
(Mar 02 2016)
ISSN: 1872-7492 [Electronic] Netherlands |
PMID | 26775814
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2016 Elsevier B.V. All rights reserved. |
Topics |
- Animals
- Animals, Newborn
- Cluster Analysis
- Computational Biology
(methods)
- Enterovirus
(classification, genetics, isolation & purification)
- Gene Expression Profiling
- Gene Expression Regulation
- Gene Ontology
- Gene Regulatory Networks
- Hand, Foot and Mouth Disease
(veterinary)
- Host-Pathogen Interactions
(genetics, immunology)
- Humans
- Immunity
(genetics)
- Leukocytes, Mononuclear
(metabolism)
- Macaca mulatta
- Molecular Sequence Annotation
- Monkey Diseases
(genetics, immunology, virology)
- Transcriptome
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