Abstract | BACKGROUND:
Puerarin, the main isoflavone glycoside extracted from the root of Pueraria lobata, is widely prescribed for patients with cardiovascular disorders in China. This study investigates the effect of puerarin on severe burn-induced acute myocardial injury in rats and its underlying mechanisms. MATERIALS AND METHODS: RESULTS: After the 30% TBSA full-thickness burn injury, serum CK-MB activities and cTnT levels increased markedly, both of which were significantly decreased by the puerarin treatment. The level of serum TNF-α concentration in burn group at each time-point was obviously higher than those in sham group (1.09±0.09 ng/ml), and it reached the peak value at 12 h post burn. Burn trauma also resulted in worsen ultrastructural condition, elevated MPO activity and MDA content in heart tissue, and a significant activation of cardiac p38 MAP kinase. Administration of puerarin improved the ultrastructural changes in cardiomyocytes, decreased TNF-α concentration in serum as well as suppressed cardiac MPO activity and reduced MDA content, and abolished the activation of p38 MAP kinase in heart tissue after severe burn. CONCLUSIONS: These results suggest that puerarin attenuates inflammatory responses, reduces neutrophil infiltration and oxidative stress in the heart, and protects against acute myocardial injury induced by severe burn.
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Authors | Sheng Liu, Hong-Bo Ren, Xu-Lin Chen, Fei Wang, Ren-Su Wang, Bo Zhou, Chao Wang, Ye-Xiang Sun, Yong-Jie Wang |
Journal | Burns : journal of the International Society for Burn Injuries
(Burns)
Vol. 41
Issue 8
Pg. 1748-1757
(Dec 2015)
ISSN: 1879-1409 [Electronic] Netherlands |
PMID | 26514700
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2015 Elsevier Ltd and ISBI. All rights reserved. |
Chemical References |
- Isoflavones
- Troponin T
- Vasodilator Agents
- Malondialdehyde
- Peroxidase
- p38 Mitogen-Activated Protein Kinases
- Creatine Kinase, MB Form
- puerarin
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Topics |
- Animals
- Burns
(complications)
- Creatine Kinase, MB Form
(blood, drug effects)
- Heart
(drug effects)
- Inflammation
- Isoflavones
(pharmacology)
- Malondialdehyde
(metabolism)
- Myocardial Ischemia
(blood, etiology)
- Myocardium
(immunology, metabolism)
- Neutrophil Infiltration
(drug effects)
- Oxidative Stress
(drug effects)
- Peroxidase
(drug effects, metabolism)
- Rats
- Rats, Wistar
- Trauma Severity Indices
- Troponin T
(blood, drug effects)
- Vasodilator Agents
(pharmacology)
- p38 Mitogen-Activated Protein Kinases
(drug effects, metabolism)
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