MiR-613: a novel diagnostic and prognostic biomarker for patients with esophageal squamous cell carcinoma.

Abstract

MicroRNA-613 (miR-613) plays important roles in tumorigenesis and cancer progression. We aimed to evaluate its expression level and potential for diagnosis and prognosis in esophageal squamous cell cancer (ESCC). We examined miR-613 expression in 60 pairs of ESCC cancerous and matched paracancerous tissues, serum samples from 75 ESCC patients and 75 healthy volunteers, and 105 formalin-fixed paraffin-embedded (FFPE) tissue samples using quantitative reverse transcription polymerase chain reaction. Receiver-operating characteristic (ROC) curve analysis, Kaplan-Meier method, and Cox regression were applied to analyze its diagnostic and prognostic value. MiR-613 was significantly decreased in ESCC tissue compared with paracancerous tissue (P < 0.001). Moreover, the expression level of miR-613 was significantly reduced with increased T stage of ESCC. Statistically significant difference between ESCC patients and healthy controls in expression level of miR-613 (0.89 ± 0.73 vs. 1.71 ± 1.03, P < 0.001) was found. The area under the ROC curve (AUC) based on serum miR-613 was 0.767 ± 0.040. We also performed analysis on early-stage patients and revealed that the AUC value was 0.728 ± 0.052 (P < 0.001). The Kaplan-Meier curve revealed that the downregulation of miR-613 was related to worse overall survival (OS) and progression-free survival (PFS) of ESCC patients (P = 0.018 and P = 0.035, respectively). Furthermore, the multivariate analysis identified miR-613 to be an independent prognostic factor for OS and PFS (P = 0.031 and P = 0.006, respectively) In conclusion, miR-613 is significantly reduced in cancerous tissue and serum samples of ESCC patients. It can serve as an ideal indicator for the diagnosis and prognosis of ESCC.

• Authors: Shanghui Guan, Cong Wang, Xuan Chen, Bowen Liu, Bingxu Tan, Fang Liu, Ding Wang, Lihui Han, Lu Wang, Xiaochen Huang, Jiangfeng Wang, Bin Yao, Jialei Shi, Pengxiang Chen, Effat Un Nesa, Qingxu Song, Yufeng Cheng
• Journal: Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine (Tumour Biol) Vol. 37 Issue 4 Pg. 4383-91 (Apr 2016) ISSN: 1423-0380 [Electronic] Netherlands
• PMID: 26499784 (Publication Type: Journal Article)

Chemical References

• Biomarkers, Tumor
• MIRN613 microRNA, human
• MicroRNAs

Topics

• Adult
• Aged
• Biomarkers, Tumor (biosynthesis, genetics)
• Carcinoma, Squamous Cell (blood, diagnosis, genetics, pathology)
• Disease-Free Survival
• Esophageal Neoplasms (blood, diagnosis, genetics, pathology)
• Esophageal Squamous Cell Carcinoma
• Female
• Gene Expression Regulation, Neoplastic
• Humans
• Kaplan-Meier Estimate
• Male
• MicroRNAs (biosynthesis, genetics)
• Middle Aged
• Neoplasm Staging
• Prognosis