Circulating Anti-Matrix Metalloproteinase-7 Antibodies May Be a Potential Biomarker for Oral Squamous Cell Carcinoma.

Abstract

PURPOSE:

The present study was conducted to evaluate the diagnostic and prognostic values of serum autoantibody against matrix metalloproteinase-7 (MMP-7) in patients with oral squamous cell carcinoma (OSCC).

MATERIALS AND METHODS:

Anti-MMP-7 antibodies were measured in sera from 204 patients with OSCC and 212 normal controls using enzyme-linked immunosorbent assay, and clinicopathologic characteristics were correlated. Prognostic consequence was assessed with Kaplan-Meier curve and log-rank tests using Cox proportional hazard models. To check whether anti-MMP-7 antibody was related to tumor associated antigen, real-time polymerase chain reaction and western blot were used to measure MMP-7 mRNA and protein expression in tumor tissues from all 204 patients with OSCC.

RESULTS:

Serum anti-MMP-7 antibody was higher in patients with OSCC (P < .05), and those with poorly differentiated tumors had more anti-MMP-7 antibody than those with well to moderate tumor differentiation (P < .01, P < .01, respectively). Patients with OSCC at late TNM stages (III, IV) and lymph node metastases had relatively higher serum anti-MMP-7 antibody levels than those with earlier stages (I, II) and those who lacked lymph node metastases (P < .05 for the 2 comparisons). OSCC prediction sensitivity as measured by receiver operating characteristics analysis was 0.485 and specificity was 0.896 (area under the curve, 0.761; 95% confidence interval, 0.716 to 0.806). Cox analysis showed that serum anti-MMP-7 antibody positivity independently predicted poor overall survival in patients with OSCC (hazard ratio, 1.82; 95% confidence interval, 1.07 to 4.61). MMP-7 mRNA and protein expression was increased in tumor tissues from patients with OSCC and high serum anti-MMP-7 antibody.

CONCLUSION:

Serum anti-MMP-7 antibody might be a novel diagnostic and prognostic biomarker for OSCC.

• Authors: Tao Jiang, Pengfeng Xie, Hongchen Liu
• Journal: Journal of oral and maxillofacial surgery : official journal of the American Association of Oral and Maxillofacial Surgeons (J Oral Maxillofac Surg) Vol. 74 Issue 3 Pg. 650-7 (Mar 2016) ISSN: 1531-5053 [Electronic] United States
• PMID: 26454036 (Publication Type: Comparative Study, Journal Article)
• Copyright: Copyright © 2016 American Association of Oral and Maxillofacial Surgeons. Published by Elsevier Inc. All rights reserved.

Chemical References

• Autoantibodies
• Biomarkers, Tumor
• MMP7 protein, human
• Matrix Metalloproteinase 7

Topics

• Aged
• Area Under Curve
• Autoantibodies (blood)
• Biomarkers, Tumor (blood)
• Carcinoma, Squamous Cell (blood, diagnosis, pathology)
• Case-Control Studies
• Electrophoresis, Polyacrylamide Gel (methods)
• Female
• Follow-Up Studies
• Humans
• Lymphatic Metastasis (pathology)
• Male
• Matrix Metalloproteinase 7 (analysis, immunology)
• Middle Aged
• Mouth Neoplasms (blood, diagnosis, pathology)
• Neoplasm Grading
• Neoplasm Staging
• Prognosis
• Proportional Hazards Models
• ROC Curve
• Retrospective Studies
• Sensitivity and Specificity
• Survival Rate