MicroRNA-590 Inhibits Lipoprotein Lipase Expression and Prevents Atherosclerosis in apoE Knockout Mice.

Abstract

Recent studies have suggested that miR-590 may play critical roles in cardiovascular disease. This study was designed to determine the effects of miR-590 on lipoprotein lipase (LPL) expression and development of atherosclerosis in apolipoprotein E knockout (apoE-/-) mice and explore the potential mechanisms. En face analysis of the whole aorta revealed that miR-590 significantly decreased aortic atherosclerotic plaque size and lipid content in apoE-/- mice. Double immunofluorescence staining in cross-sections of the proximal aorta showed that miR-590 agomir reduced CD68 and LPL expression in macrophages in atherosclerotic lesions. MiR-590 agomir down-regulated LPL mRNA and protein expression as analyzed by RT-qPCR and western blotting analyses, respectively. Consistently, miR-590 decreased the expression of CD36 and scavenger receptor A1 (SRA1) mRNA and protein. High-performance liquid chromatography (HPLC)analysis confirmed that treatment with miR-590 agomir reduced lipid levels either in plasma orinabdominal cavity macrophages of apoE-/- mice. ELISA analysis showed that miR-590 agomir decreased plasma levels of pro-inflammatory cytokines, such as tumor necrosis factor-alpha (TNF-α), monocyte chemotactic protein-1 (MCP-1), interleukin-1β (IL-1β)and interleukin-6 (IL-6). In contrast, treatment with miR-590 antagomir prevented or reversed these effects. Taken together, these results reveal a novel mechanism of miR-590 effects, and may provide new insights into the development of strategies for attenuating lipid accumulation and pro-inflammatory cytokine secretion.

• Authors: Ping-Ping He, Xin-Ping OuYang, Yuan Li, Yun-Cheng Lv, Zong-Bao Wang, Feng Yao, Wei Xie, Yu-Lin Tan, Liang Li, Min Zhang, Gang Lan, Duo Gong, Hai-Peng Cheng, Hui-Juan Zhong, Dan Liu, Chong Huang, Zhao-Xia Li, Xi-Long Zheng, Wei-Dong Yin, Chao-Ke Tang
• Journal: PloS one (PLoS One) Vol. 10 Issue 9 Pg. e0138788 ( 2015) ISSN: 1932-6203 [Electronic] United States
• PMID: 26397958 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Apolipoproteins E
• CD36 Antigens
• Cytokines
• MIRN590 microRNA, mouse
• MicroRNAs
• Lipoprotein Lipase

Topics

• Animals
• Aorta (enzymology, pathology)
• Apolipoproteins E (genetics)
• Atherosclerosis (enzymology)
• CD36 Antigens (metabolism)
• Cytokines (blood)
• Enzyme Repression
• Lipid Metabolism
• Lipoprotein Lipase (genetics, metabolism)
• Macrophages, Peritoneal (enzymology)
• Male
• Mice, Knockout
• MicroRNAs (genetics, metabolism)
• RNA Interference