Effects of active immunisation with myelin basic protein and myelin-derived altered peptide ligand on pain hypersensitivity and neuroinflammation.

Neuropathic pain is a debilitating condition in multiple sclerosis and experimental autoimmune encephalomyelitis (EAE). Specific myelin basic protein (MBP) peptides are encephalitogenic, and myelin-derived altered peptide ligands (APLs) are capable of preventing and ameliorating EAE. We investigated the effects of active immunisation with a weakly encephalitogenic epitope of MBP (MBP87-99) and its mutant APL (Cyclo-87-99[A(91),A(96)]MBP87-99) on pain hypersensitivity and neuroinflammation in Lewis rats. MBP-treated rats exhibited significant mechanical and thermal pain hypersensitivity associated with infiltration of T cells, MHC class II expression and microglia activation in the spinal cord, without developing clinical signs of paralysis. Co-immunisation with APL significantly decreased pain hypersensitivity and neuroinflammation emphasising the important role of neuroimmune crosstalk in neuropathic pain.
AuthorsChamini J Perera, Justin G Lees, Samuel S Duffy, Preet G S Makker, Brett Fivelman, Vasso Apostolopoulos, Gila Moalem-Taylor
JournalJournal of neuroimmunology (J Neuroimmunol) Vol. 286 Pg. 59-70 (Sep 15 2015) ISSN: 1872-8421 [Electronic] Netherlands
PMID26298325 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
CopyrightCopyright © 2015 Elsevier B.V. All rights reserved.
Chemical References
  • Histocompatibility Antigens Class II
  • Ligands
  • Myelin Basic Protein
  • Peptide Fragments
  • myelin basic protein 85-99
  • Freund's Adjuvant
  • Animals
  • Disease Models, Animal
  • Exploratory Behavior (drug effects)
  • Freund's Adjuvant (toxicity)
  • Histocompatibility Antigens Class II (metabolism)
  • Humans
  • Hypersensitivity (etiology, metabolism)
  • Ligands
  • Lymphocyte Activation (immunology)
  • Male
  • Microglia (drug effects, metabolism)
  • Myelin Basic Protein (immunology, toxicity)
  • Myelitis (chemically induced, complications, immunology)
  • Pain (chemically induced, physiopathology)
  • Pain Measurement
  • Pain Threshold
  • Peptide Fragments (immunology, toxicity)
  • Rats
  • Rats, Inbred Lew
  • Spinal Cord (metabolism, pathology)
  • T-Lymphocytes (drug effects, metabolism)
  • Time Factors
  • Vaccination (adverse effects)

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