Vaccination with different doses of Dermatophagoides farinae in a mouse model of allergic asthma.

Abstract

BACKGROUND:

The efficacy of vaccination with different doses of a vaccine has not been clarified. The purpose of this study was to investigate the relationship between the doses of purified house dust mite allergen, and allergic airway inflammation and airway reactivity in a mouse model of allergic asthma.

METHODS:

C57BL/6 mice were sensitized with Dermatophagoides farinae (Der f) and randomly treated with saline, 0.1, 1, or 2 mg Der f vaccine, respectively. The airway hyperresponsiveness, the numbers of total cells and eosinophils and the levels of interferon-γ and interleukin (IL)-4 in the bronchoalveolar lavage fluid (BALF) from individual mice were measured. The percentages of CD11c CD86 and CD11c MHC II+ dendritic cells were characterized, and the levels of IL-12 in the supernatants of cultured dendritic cells were measured.

RESULTS:

The airway hyperresponsiveness, the numbers of total cells and eosinophils in the BALF and the percentages of CD11c CD86 and CD11c MHC II+ dendritic cells were associated with immunologic downregulation. The levels of IFN-γ in the BALF and the levels of IL-12 in the supernatants of cultured dendritic cells were associated with immunologic upregulation. It was found that vaccination with the medium dose of Der f showed a stronger inhibitory effect.

CONCLUSIONS:

There seems to be a dose-dependent response between allergen and both airway inflammation and hyperreactivity in the mouse model of allergic asthma.

• Authors: Jie Han, Ying Huang, Xue Jun Wu, Mu-Kui Wang
• Journal: The American journal of the medical sciences (Am J Med Sci) Vol. 350 Issue 2 Pg. 116-20 (Aug 2015) ISSN: 1538-2990 [Electronic] United States
• PMID: 26226406 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antigens, CD
• Antigens, Dermatophagoides
• Vaccines
• Interleukin-12

Topics

• Animals
• Antigens, CD (immunology)
• Antigens, Dermatophagoides (administration & dosage)
• Asthma (prevention & control)
• Bronchoalveolar Lavage Fluid
• Dendritic Cells (immunology, metabolism)
• Dermatophagoides farinae (immunology)
• Dose-Response Relationship, Immunologic
• Enzyme-Linked Immunosorbent Assay
• Female
• Flow Cytometry
• Hypersensitivity (prevention & control)
• Interleukin-12 (metabolism)
• Mice
• Mice, Inbred C57BL
• Vaccines (administration & dosage, immunology)