Inhibition of Recombining Binding Protein Suppressor of Hairless (RBPJ) Impairs the Growth of Prostate Cancer.

Abstract

BACKGROUND/AIMS:

Notch signaling pathway regulates cancer cell growth. RBPJ is a key transcription factor downstream of Notch receptor activation, whereas the role of RBPJ in carcinogenesis of prostate cancer is ill-defined.

METHODS:

Here, we evaluated the effects of RBPJ inhibition on the growth of prostate cancer cells. We knocked down RBPJ in prostate cancer cells by a short hairpin interfering RNA (shRNA). We measured cell growth by an MTT assay. We analyzed the levels of cell-cycle-associated proteins by Western blot.

RESULTS:

We found that shRNA for RBPJ efficiently inhibited RBPJ expression in prostate cancer cells, resulting in a significant decrease in the cell growth. Further, RBPJ-mediated cell-growth inhibition appeared to be resulting from alteration of cell-cycle inhibitors p21 and p27, cell-cycle activators CDK2, CDK4 and CyclinD1, and apoptosis-suppressor Bcl-2.

CONCLUSION:

Our data suggest that shRNA intervention of RBPJ expression could be a promising therapeutic approach for treating human prostate cancer.

• Authors: Li Xue, Hecheng Li, Qi Chen, Zhenlong Wang, Peng Zhang, Haiwen Chen, Ziming Wang, Tie Chong
• Journal: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology (Cell Physiol Biochem) Vol. 36 Issue 5 Pg. 1982-90 ( 2015) ISSN: 1421-9778 [Electronic] Germany
• PMID: 26202358 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2015 S. Karger AG, Basel.

Chemical References

• Immunoglobulin J Recombination Signal Sequence-Binding Protein
• RBPJ protein, human
• RNA, Small Interfering

Topics

• Cell Line, Tumor
• Cell Proliferation (genetics)
• HEK293 Cells
• Humans
• Immunoglobulin J Recombination Signal Sequence-Binding Protein (genetics)
• Male
• Prostatic Neoplasms (genetics, pathology)
• RNA, Small Interfering (genetics)