Abstract | CONTEXT: OBJECTIVE: The anti-inflammatory activity from the N. mirabilis methanolic extract led to the isolation of compounds (1-26). MATERIALS AND METHODS: Chromatographic methods were used to isolate compounds from the methanol extract of N. mirabilis branches and leaves. The anti-inflammatory activity of these isolated compounds was investigated in lipopolysaccharide (LPS)-stimulated bone marrow-derived dendritic cells (BMDCs) using ELISA. Primary BMDCs were used to examine the production of pro-inflammatory cytokines ( IL-12 p40, IL-6, and TNF-α, at concentrations of 0.1, 0.2, and 1.0 μM) as compared with a positive control, SB203580 (1.0 μM). MTT assays showed that isolated compounds (1-26) did not exhibit significant cytotoxicity at concentrations up to 20.0 μM. RESULTS: Compound 9 showed potent inhibition of IL-12 p40, IL-6, and TNF-α production (IC50 = 0.17 ± 0.02, 0.46 ± 0.01, and 8.28 ± 0.21 μM, respectively). Compound 4 showed potent inhibition of IL-12 p40 and IL-6 production (IC50 = 1.17 ± 0.01 and 2.15 ± 0.04 μM). In addition, IL-12 p40 inhibition by naphthalene derivatives (1-7, 9, and 10), phenolic compounds (11-15), lupeone (18), and flavonoids (22, 25, and 26) was more potent than with the positive control. The isolated compounds exhibited little and/or no inhibitory effects on TNF-α production in LPS-stimulated BMDCs. DISCUSSION AND CONCLUSION: Taken together, these data suggest that the isolated components have significant inhibitory effects on pro-inflammatory cytokine production and warrant further study concerning their potential medicinal use.
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Authors | Nguyen Phuong Thao, Bui Thi Thuy Luyen, Jung Eun Koo, Sohyun Kim, Young Sang Koh, Nguyen Van Thanh, Nguyen Xuan Cuong, Phan Van Kiem, Chau Van Minh, Young Ho Kim |
Journal | Pharmaceutical biology
(Pharm Biol)
Vol. 54
Issue 4
Pg. 588-94
( 2016)
ISSN: 1744-5116 [Electronic] England |
PMID | 26186458
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Anti-Inflammatory Agents
- Inflammation Mediators
- Plant Extracts
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Topics |
- Anti-Inflammatory Agents
(chemistry, isolation & purification, pharmacology)
- Bone Marrow Cells
(drug effects, physiology)
- Cell Survival
(drug effects, physiology)
- Cells, Cultured
- Humans
- Inflammation Mediators
(antagonists & inhibitors, physiology)
- Mirabilis
- Plant Extracts
(chemistry, isolation & purification, pharmacology)
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