Abstract | BACKGROUND: METHODS: Ovarian theca interna tissues were obtained from 10 PCOS patients and 8 controls that were non-PCOS and had normal insulin sensitivity undergoing laparoscopy and/or ovarian wedge resection. Total RNA of all samples was extracted. We studied the repertoire of miRNAs in both PCOS and non-PCOS women by microarray hybridization. Bioinformatic analysis was performed for predicting targets of the differentially expressed miRNAs. Furthermore, selected miRNAs were validated by quantitative reverse transcriptase polymerase chain reaction (qRT-PCR). RESULTS: A total of 27 miRNAs were differentially expressed in PCOS patients with respect to the controls in our discovery evaluationand two (miR-92a and miR-92b) of them were significantly downregulated in PCOS women in followed validation (P < 0.05). Targets prediction revealed that miR-92a targeted both GATA family of zinc finger transcription factor GATA-binding factor 6 (GATA6) and insulin receptor substrate proteins 2 (IRS-2). CONCLUSIONS:
MiRNAs are differentially expressed between PCOS patients and controls. We identified and validated two miRNAs-miR-92a and miR-92b. They are significantly downregulated and may be involved in the pathogenesis of PCOS.
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Authors | Lin Lin, Tao Du, Jia Huang, Li-Li Huang, Dong-Zi Yang |
Journal | Chinese medical journal
(Chin Med J (Engl))
Vol. 128
Issue 2
Pg. 169-74
(Jan 20 2015)
ISSN: 2542-5641 [Electronic] China |
PMID | 25591557
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
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Topics |
- Adult
- Female
- Humans
- Hyperandrogenism
(genetics)
- Insulin Resistance
(genetics, physiology)
- Male
- MicroRNAs
(genetics)
- Ovary
(metabolism)
- Polycystic Ovary Syndrome
(genetics)
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