Increased serum ADAMTS-4 in knee osteoarthritis: a potential indicator for the diagnosis of osteoarthritis in early stages.

Abstract

We compared serum levels of a disintegrin and metalloproteinase with thrombospondin motifs (ADAMTS)-4, ADAMTS-5, matrix metalloproteinase (MMP)-1, and MMP-3 in patients with different stages of knee osteoarthritis (OA), and investigated the clinical significance of diagnosing OA in early stages. OA patients were divided into 2 groups: early OA group (44 cases), intermediate and advanced OA group (26 cases). The healthy control group included 30 samples. ADAMTS-4, ADAMTS-5, MMP-1, and MMP-3 levels in the serum were tested using an enzyme-linked immunosorbent assay. A protein-protein interaction network was constructed by seeding the significantly expressed marker, followed by Gene Ontology enrichment analyses using Database for Annotation, Visualization and Integrated Discovery. ADAMTS-4 levels were significantly higher in patients at early stages of OA compared to intermediate or advanced OA and healthy controls. ADAMTS-5, MMP-1, and MMP-3 levels in intermediate and advanced-stage OA patients were significantly higher than those in early-stage OA patients and healthy controls. The protein-protein interaction network showed that ADAMTS-4 participates in 67 interactions. Gene Ontology enrichment analysis validated that genes associated with ADAMTS-4 participate in collagen metabolism and OA. ADAMTS-4 is a potential biomarker as an early diagnostic indicator of OA.

• Authors: W Li, C Du, H Wang, C Zhang
• Journal: Genetics and molecular research : GMR (Genet Mol Res) Vol. 13 Issue 4 Pg. 9642-9 (Nov 14 2014) ISSN: 1676-5680 [Electronic] Brazil
• PMID: 25501175 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Biomarkers
• Collagen
• ADAM Proteins
• Procollagen N-Endopeptidase
• Matrix Metalloproteinase 3
• Matrix Metalloproteinase 1
• ADAMTS4 Protein
• ADAMTS4 protein, human

Topics

• ADAM Proteins (blood, genetics)
• ADAMTS4 Protein
• Biomarkers (blood)
• Case-Control Studies
• Collagen (genetics, metabolism)
• Disease Progression
• Early Diagnosis
• Gene Expression
• Gene Regulatory Networks
• Humans
• Matrix Metalloproteinase 1 (blood, genetics)
• Matrix Metalloproteinase 3 (blood, genetics)
• Osteoarthritis, Knee (blood, diagnosis, genetics, pathology)
• Procollagen N-Endopeptidase (blood, genetics)
• Protein Interaction Mapping
• Severity of Illness Index