Abstract | AIMS:
Perilipin-1 (Plin1), exclusively located on the surface of lipid droplets in adipocytes, regulates the storage and hydrolysis of adipose triglycerides. Plin1 deficiency primarily causes low adiposity and aberrant lipolysis in rodents and humans. Here, we investigated whether adipose tissue dysfunction in perilipin-1 null (Plin1⁻/⁻) mice has maladaptive consequences for the heart and an association with hypertrophic cardiomyopathy. METHODS AND RESULTS:
Perilipin-1 was expressed specifically in adipocytes but was undetectable in cardiomyocytes. Plin1⁻/⁻ mice were histologically lipodystrophic, with reduced body fat. Paradoxically, the adipocytes of Plin1⁻/⁻ mice, like those of obese and diabetic mammals, showed robust basal lipolysis and fatty acid efflux to the plasma. Such adipose tissue dysfunctions accounted for the ectopic lipid accumulation and enhanced fatty acid transport and oxidation in Plin1⁻/⁻ mouse hearts. Excessive fatty acid β-oxidation and lipotoxicity induced excessive production of reactive oxygen species and oxidative stress because antioxidative capacity was reduced in cardiomyocytes, These malefactors injured the myocardial structure and function, as evidenced by disorganized myofilaments as well as irregular and swollen mitochondria with disrupted cristae. Finally, Plin1⁻/⁻ mice showed grossly visible cardiac hypertrophy, with progressively up-regulated expression of hypertrophy and dysfunction marker genes, leading to heart failure, particularly with left ventricular diastolic dysfunction at 20 weeks of age. CONCLUSIONS:
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Authors | Shangxin Liu, Bin Geng, Liangqiang Zou, Suning Wei, Weiyi Wang, Jingna Deng, Chong Xu, Xiaojing Zhao, Ying Lyu, Xueying Su, Guoheng Xu |
Journal | Cardiovascular research
(Cardiovasc Res)
Vol. 105
Issue 1
Pg. 20-30
(Jan 01 2015)
ISSN: 1755-3245 [Electronic] England |
PMID | 25416668
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Carrier Proteins
- Fatty Acids
- PLIN1 protein, human
- Perilipin-1
- Phosphoproteins
- Plin1 protein, mouse
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Topics |
- Adipose Tissue
(physiopathology)
- Animals
- Cardiomyopathy, Hypertrophic
(etiology, genetics, physiopathology)
- Carrier Proteins
(genetics, physiology)
- Disease Models, Animal
- Fatty Acids
(metabolism)
- Lipid Metabolism
- Lipolysis
- Male
- Mice
- Mice, 129 Strain
- Mice, Knockout
- Myocytes, Cardiac
(metabolism, pathology)
- Oxidative Stress
- Perilipin-1
- Phosphoproteins
(deficiency, genetics, physiology)
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