Berteroin present in cruciferous vegetables exerts potent anti-inflammatory properties in murine macrophages and mouse skin.

Abstract

Berteroin (5-methylthiopentyl isothiocyanate) is a sulforaphane analog present in cruciferous vegetables, including Chinese cabbage, rucola salad leaves, and mustard oil. We examined whether berteroin exerts anti-inflammatory activities using lipopolysaccharide (LPS)-stimulated Raw 264.7 macrophages and 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced mouse skin inflammation models. Berteroin decreased LPS-induced release of inflammatory mediators and pro-inflammatory cytokines in Raw 264.7 macrophages. Berteroin inhibited LPS-induced degradation of inhibitor of κBα (IκBα) and nuclear factor-κB p65 translocation to the nucleus and DNA binding activity. Furthermore, berteroin suppressed degradation of IL-1 receptor-associated kinase and phosphorylation of transforming growth factor β activated kinase-1. Berteroin also inhibited LPS-induced phosphorylation of p38 MAPK, ERK1/2, and AKT. In the mouse ear, berteroin effectively suppressed TPA-induced edema formation and down-regulated iNOS and COX-2 expression as well as phosphorylation of AKT and ERK1/2. These results demonstrate that berteroin exhibits potent anti-inflammatory properties and suggest that berteroin can be developed as a skin anti-inflammatory agent.

• Authors: Yoo Jin Jung, Jae In Jung, Han Jin Cho, Myung-Sook Choi, Mi-Kyung Sung, Rina Yu, Young-Hee Kang, Jung Han Yoon Park
• Journal: International journal of molecular sciences (Int J Mol Sci) Vol. 15 Issue 11 Pg. 20686-705 (Nov 11 2014) ISSN: 1422-0067 [Electronic] Switzerland
• PMID: 25393510 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Anti-Inflammatory Agents
• Cytokines
• I-kappa B Proteins
• Isothiocyanates
• Lipopolysaccharides
• Nfkbia protein, mouse
• berteroin
• NF-KappaB Inhibitor alpha
• Nitric Oxide
• 4-O-methyl-12-O-tetradecanoylphorbol 13-acetate
• Nitric Oxide Synthase Type II
• Cyclooxygenase 2
• Tetradecanoylphorbol Acetate

Topics

• Animals
• Anti-Inflammatory Agents (chemistry, pharmacology)
• Cyclooxygenase 2 (immunology)
• Cytokines (immunology)
• Female
• I-kappa B Proteins (immunology)
• Inflammation (chemically induced, drug therapy, immunology)
• Isothiocyanates (chemistry, pharmacology)
• Lipopolysaccharides (immunology)
• Macrophages (drug effects, immunology)
• Mice
• NF-KappaB Inhibitor alpha
• Nitric Oxide (immunology)
• Nitric Oxide Synthase Type II (immunology)
• Skin (drug effects, immunology)
• Tetradecanoylphorbol Acetate (analogs & derivatives)
• Vegetables (chemistry)