Abstract | PURPOSE: METHODS:
Chondroitin sulfate proteoglycan mimics, designed to bind to type II collagen, hyaluronic acid, or both, were applied to trypsin- or collagenase-treated bovine vitreous in situ and in vitro. Rheology and liquefaction tests were performed to determine the physical properties of the vitreous, while Western blots were used to detect the presence and degradation of soluble collagen II (α1). Deep-etch electron microscopy (DEEM) identified the ultrastructure of mimic-treated and untreated enzyme-degraded bovine vitreous. RESULTS:
Proteoglycan mimics preserved the physical properties of trypsin-degraded bovine vitreous and protected against vitreous liquefaction. Although the collagen-binding mimic maintained the physical properties of collagenase-treated vitreous, liquefaction still occurred. Western blots indicated that the mimic provided only marginal protective ability against soluble collagen degradation. Deep-etch electron microscopy, however, showed increased density and isotropy of microstructural components in mimic-treated vitreous, supporting the initial result that vitreous structure was preserved. CONCLUSIONS:
Proteoglycan mimics preserved bovine vitreous physical properties after enzymatic degradation. These compounds may be useful in delaying or preventing the pathological effects of age-related, or enzymatically-induced, degradation of the vitreous body.
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Authors | Qianru Zhang, Benjamen A Filas, Robyn Roth, John Heuser, Nan Ma, Shaili Sharma, Alyssa Panitch, David C Beebe, Ying-Bo Shui |
Journal | Investigative ophthalmology & visual science
(Invest Ophthalmol Vis Sci)
Vol. 55
Issue 12
Pg. 8153-62
(Oct 23 2014)
ISSN: 1552-5783 [Electronic] United States |
PMID | 25342623
(Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
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Copyright | Copyright 2014 The Association for Research in Vision and Ophthalmology, Inc. |
Chemical References |
- Chondroitin Sulfate Proteoglycans
- Collagen Type II
- Hyaluronic Acid
- Collagenases
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Topics |
- Animals
- Blotting, Western
- Cattle
- Chondroitin Sulfate Proteoglycans
(chemistry, pharmacology)
- Collagen Type II
(analysis)
- Collagenases
(pharmacology)
- Elasticity
(physiology)
- Hyaluronic Acid
(metabolism)
- Microscopy, Electron
- Retinal Diseases
(drug therapy, physiopathology)
- Vitreous Body
(drug effects, physiology, ultrastructure)
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