Sargachromenol from Sargassum micracanthum inhibits the lipopolysaccharide-induced production of inflammatory mediators in RAW 264.7 macrophages.

Abstract

During our ongoing screening program designed to determine the anti-inflammatory potential of natural compounds, we isolated sargachromenol from Sargassum micracanthum. In the present study, we investigated the anti-inflammatory effects of sargachromenol on lipopolysaccharide (LPS)-induced inflammation in murine RAW 264.7 macrophage cells and the underlying mechanisms. Sargachromenol significantly inhibited the LPS-induced production of nitric oxide (NO) and prostaglandin E₂ (PGE₂) in a dose-dependent manner. It also significantly inhibited the protein expression of inducible NO synthase (iNOS) and cyclooxygenase-2 (COX-2) in a dose-dependent manner in LPS-stimulated macrophage cells. Further analyses showed that sargachromenol decreased the cytoplasmic loss of inhibitor κBα (IκBα) protein. These results suggest that sargachromenol may exert its anti-inflammatory effects on LPS-stimulated macrophage cells by inhibiting the activation of the NF-κB signaling pathway. In conclusion, to our knowledge, this is the first study to show that sargachromenol isolated from S. micracanthum has an effective anti-inflammatory activity. Therefore, sargachromenol might be useful for cosmetic, food, or medical applications requiring anti-inflammatory properties.

• Authors: Eun-Jin Yang, Young Min Ham, Kyong-Wol Yang, Nam Ho Lee, Chang-Gu Hyun
• Journal: TheScientificWorldJournal (ScientificWorldJournal) Vol. 2013 Pg. 712303 ( 2013) ISSN: 1537-744X [Electronic] United States
• PMID: 24194688 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Anti-Inflammatory Agents
• Benzopyrans
• Inflammation Mediators
• Lipopolysaccharides
• sargachromenol
• Nitric Oxide
• Dinoprostone

Topics

• Animals
• Anti-Inflammatory Agents (administration & dosage)
• Benzopyrans (administration & dosage, metabolism)
• Cell Line
• Dinoprostone (immunology)
• Inflammation (chemically induced, immunology, prevention & control)
• Inflammation Mediators (immunology)
• Lipopolysaccharides
• Macrophage Activation (drug effects, immunology)
• Macrophages (drug effects, immunology)
• Mice
• Nitric Oxide (immunology)
• Sargassum (metabolism)