Octaphlorethol A isolated from Ishige foliacea inhibits α-MSH-stimulated induced melanogenesis via ERK pathway in B16F10 melanoma cells.

Abstract

In this study, the potent skin-whitening effects of Octaphlorethol A (OPA) isolated from Ishige foliacea was investigated through inhibitory effect of melanin synthesis and tyrosinase activity in alpha-melanocyte stimulating hormone (α-MSH) induced B16F10 melanoma cells. OPA markedly inhibited melanin synthesis and tyrosinase activity in a concentration-dependent manner. We also found that OPA decreased microphthalmia-associated transcription factor (MITF), tyrosinase, tyrosinase-related protein-1 and -2 (TRP-1 and TRP-2) protein expressions. Moreover, OPA reduces p38 MAPK protein levels and activates extracellular signal-regulated kinase (ERK) and c-jun N-terminal kinases (JNKs) protein expressions in B16F10 cells. A specific ERK inhibitor PD98059 significantly blocks OPA-inhibited melanin synthesis and tyrosinase activity, whereas a p38MAP and JNK inhibitor had no effect. These findings provide evidence demonstrating that the anti-melanogenic effect of OPA is mediated through the activation of ERK signal pathway in B16F10 cells. These results indicate that OPA has the potential to be used as a melanogenesis inhibitor in the food and cosmetics industry.

• Authors: Kil-Nam Kim, Hye-Mi Yang, Sung-Myung Kang, Daekyung Kim, Ginnae Ahn, You-Jin Jeon
• Journal: Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association (Food Chem Toxicol) Vol. 59 Pg. 521-6 (Sep 2013) ISSN: 1873-6351 [Electronic] England
• PMID: 23810793 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2013 Elsevier Ltd. All rights reserved.

Chemical References

• Melanins
• Membrane Glycoproteins
• Microphthalmia-Associated Transcription Factor
• Mitf protein, mouse
• Neoplasm Proteins
• Phenols
• Protein Kinase Inhibitors
• Skin Lightening Preparations
• octaphlorethol A
• alpha-MSH
• Oxidoreductases
• Tyrp1 protein, mouse
• Monophenol Monooxygenase
• Intramolecular Oxidoreductases
• dopachrome isomerase

Topics

• Animals
• Cell Line, Tumor
• Cell Survival (drug effects)
• Down-Regulation (drug effects)
• Drug Discovery
• Intramolecular Oxidoreductases (antagonists & inhibitors, metabolism)
• MAP Kinase Signaling System (drug effects)
• Melanins (antagonists & inhibitors, biosynthesis)
• Melanocytes (drug effects, enzymology, metabolism)
• Membrane Glycoproteins (antagonists & inhibitors, metabolism)
• Mice
• Microphthalmia-Associated Transcription Factor (antagonists & inhibitors, metabolism)
• Monophenol Monooxygenase (antagonists & inhibitors, metabolism)
• Neoplasm Proteins (antagonists & inhibitors, biosynthesis, metabolism)
• Oxidoreductases (antagonists & inhibitors, metabolism)
• Phaeophyceae (chemistry)
• Phenols (adverse effects, antagonists & inhibitors, isolation & purification, pharmacology)
• Pigmentation (drug effects)
• Protein Kinase Inhibitors (pharmacology)
• Skin Lightening Preparations (adverse effects, chemistry, isolation & purification, pharmacology)
• alpha-MSH (antagonists & inhibitors, metabolism)