Abstract | ETHNOPHARMACOLOGICAL RELEVANCE: Hymenaea courbaril L. (Caesalpinoideae) is used in Brazilian folk medicine to treat anemia, kidney problems, sore throat and other dysfunctions of the respiratory system, such as bronchitis and asthma, although such properties are yet to be scientifically validated. AIM OF THE STUDY: In order to give a scientific basis to support the traditional use of Hymenaea courbaril, this study was designed to evaluate antioxidant, myorelaxant and anti-inflammatory properties of the ethanol extract from stem bark and its fractions. The myorelaxant effect of astilbin, a flavonoid isolated from the bioactive ethyl acetate fraction (EAF), has also been evaluated. MATERIAL AND METHODS: RESULTS: The results of the present investigation show that the whole extract of Hymenaea courbaril and some of its fractions strongly scavenged DPPH radical. The extract showed myorelaxant activity on rat trachea, being EAF its highest efficient fraction. Bio-guided study allowed the isolation of astilbin, a well-known flavonoid. The activity induced by this compound indicates that it may be partly responsible for the myorelaxant effect of EAF. EAF reduced contractions that depended on divalent cation inflow through voltage-operated Ca(2+) channels (VOCCs) or receptor-operated Ca(2+) channels (ROCCs), but it was more potent to inhibit VOCC- than ROCC-dependent contraction induced by Ca(2+) addition in ACh-enriched Ca(2+)-free medium. Oral pretreatment of antigen-challenged animals with EAF prevented airway hyperresponsiveness on KCl-induced contraction and reduced the number of total white cells, particularly eosinophils and neutrophils in bronchoalveolar lavage. CONCLUSIONS: This study provided scientific basis that Hymenaea courbaril presents potential antioxidant, myorelaxant and anti-inflammatory actions, which support its use in folk medicine to treat inflammatory airway diseases.
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Authors | Gabrieli Penha Bezerra, Roberto Wagner da Silva Góis, Teresinha Silva de Brito, Francisco José Batista de Lima, Mary Anne Medeiros Bandeira, Nirla Rodrigues Romero, Pedro Jorge Caldas Magalhães, Gilvandete Maria Pinheiro Santiago |
Journal | Journal of ethnopharmacology
(J Ethnopharmacol)
Vol. 149
Issue 1
Pg. 62-9
(Aug 26 2013)
ISSN: 1872-7573 [Electronic] Ireland |
PMID | 23764737
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2013 Elsevier Ireland Ltd. All rights reserved. |
Chemical References |
- Anti-Inflammatory Agents
- Antioxidants
- Plant Extracts
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Topics |
- Animals
- Anti-Inflammatory Agents
(isolation & purification, pharmacology, therapeutic use)
- Antioxidants
(isolation & purification, pharmacology, therapeutic use)
- Brazil
- Chemical Fractionation
- Dose-Response Relationship, Drug
- Ethnopharmacology
(methods)
- Hymenaea
(chemistry, growth & development)
- In Vitro Techniques
- Male
- Medicine, Traditional
- Muscle Relaxation
(drug effects)
- Plant Bark
(chemistry)
- Plant Extracts
(isolation & purification, pharmacology, therapeutic use)
- Plant Stems
(chemistry)
- Rats
- Rats, Wistar
- Respiratory Hypersensitivity
(drug therapy, immunology)
- Trachea
(drug effects)
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