Correlation of acute phase inflammatory and oxidative markers with long-term cognitive impairment in sepsis survivors rats.

Abstract

Oxidative damage and inflammation occur early in the brain after sepsis and are resolved when long-term cognitive impairment occurs. There is no information of a direct relation between acute levels of brain inflammation and oxidative damage and long-term cognitive deficits. We hypothesized that higher levels of early oxidative damage and inflammation are followed by long-term cognitive deficits, and this is related to a decrease in the levels of brain-derived neurotropic factor (BDNF). Wistar rats were subjected to sham operation or cecal ligation and perforation and the cerebrospinal fluid (CSF) was obtained 6 and 24 h after the determination of thiobarbituric acid-reactive species, interleukin 1 (IL-1), IL-10, and tumor necrosis factor α (TNF-α). Animals were followed until 30 days after surgery and were subjected to the step-down inhibitory avoidance (IA) task, and the hippocampus levels of BDNF were determined. At 6 h, higher CSF levels of thiobarbituric acid-reactive species and TNF-α were observed in septic animals that had a better performance in the IA task and presented higher BDNF levels in the hippocampus. At 24 h, higher CSF levels of IL-1β and TNF-α were observed in septic animals that had a worse performance in the IA task, and this was associated with lower BDNF levels. The persistence of brain inflammation during the acute phase of sepsis is associated with long-term hippocampus levels of BDNF and memory impairment in sepsis survivors.

• Authors: Daiane Biff, Fabrícia Petronilho, Larissa Constantino, Francieli Vuolo, Grettel J Zamora-Berridi, Dhébora Mozena Dall'Igna, Clarissa M Comim, João Quevedo, Flávio Kapczinski, Felipe Dal-Pizzol
• Journal: Shock (Augusta, Ga.) (Shock) Vol. 40 Issue 1 Pg. 45-8 (Jul 2013) ISSN: 1540-0514 [Electronic] United States
• PMID: 23603768 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Interleukin-1
• Thiobarbituric Acid Reactive Substances
• Tumor Necrosis Factor-alpha
• Interleukin-10

Topics

• Animals
• Cognition Disorders (metabolism)
• Enzyme-Linked Immunosorbent Assay
• Interleukin-1 (metabolism)
• Interleukin-10 (metabolism)
• Male
• Rats
• Rats, Wistar
• Sepsis (metabolism, physiopathology)
• Thiobarbituric Acid Reactive Substances (metabolism)
• Tumor Necrosis Factor-alpha (metabolism)