Glucan-rich polysaccharides from Pleurotus sajor-caju (Fr.) Singer prevents glucose intolerance, insulin resistance and inflammation in C57BL/6J mice fed a high-fat diet.

Abstract

BACKGROUND:

Pleurotus sajor-caju (P. sajor-caju) has been extremely useful in the prevention of diabetes mellitus due to its low fat and high soluble fiber content for thousands of years. Insulin resistance is a key component in the development of diabetes mellitus which is caused by inflammation. In this study, we aimed to investigate the in vivo efficacy of glucan-rich polysaccharide of P. sajor-caju (GE) against diabetes mellitus and inflammation in C57BL/6J mice fed a high-fat diet.

METHODS:

Diabetes was induced in C57BL/6J mice by feeding a high-fat diet. The mice were randomly assigned to 7 groups (n=6 per group). The control groups in this study were ND (for normal diet) and HFD (for high-fat diet). The treated groups were ND240 (for normal diet) (240 mg/kg b.w) and HFD60, HFD120 and HFD240 (for high-fat), where the mice were administrated with three dosages of GE (60, 120, 240 mg GE/kg b.w respectively). Metformin (2 mg/kg b.w) served as positive control. The glucose tolerance test, glucose and insulin levels were measured at the end of 16 weeks. Expressions of genes for inflammatory markers, GLUT-4 and adiponectin in the adipose tissue of the mice were assessed. One-way ANOVA and Duncan's multiple range tests (DMRT) were used to determine the significant differences between groups.

RESULTS:

GE treated groups improved the glucose tolerance, attenuated hyperglycemia and hyperinsulinemia in the mice by up-regulating the adiponectin and GLUT-4 gene expressions. The mice in GE treated groups did not develop insulin resistance. GE also down-regulated the expression of inflammatory markers (IL-6, TNF-α, SAA2, CRP and MCP-1) via attenuation of nuclear transcription factors (NF-κB).

CONCLUSION:

Glucan-rich polysaccharide of P. sajor-caju can serve as a potential agent for prevention of glucose intolerance, insulin resistance and inflammation.

• Authors: Gowri Kanagasabapathy, Umah Rani Kuppusamy, Sri Nurestri Abd Malek, Mahmood Ameen Abdulla, Kek-Heng Chua, Vikineswary Sabaratnam
• Journal: BMC complementary and alternative medicine (BMC Complement Altern Med) Vol. 12 Pg. 261 (Dec 21 2012) ISSN: 1472-6882 [Electronic] England
• PMID: 23259700 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Blood Glucose
• Ccl2 protein, mouse
• Chemokine CCL2
• Dietary Fats
• Glucans
• Insulin
• Interleukin-6
• NF-kappa B
• Polysaccharides
• Tumor Necrosis Factor-alpha

Topics

• Animals
• Blood Glucose (metabolism)
• Chemokine CCL2 (genetics, immunology)
• Dietary Fats (metabolism)
• Female
• Fruiting Bodies, Fungal (chemistry)
• Functional Food (analysis)
• Gene Expression (drug effects)
• Glucans (administration & dosage, analysis)
• Glucose Intolerance (drug therapy, immunology, metabolism, prevention & control)
• Humans
• Insulin (metabolism)
• Insulin Resistance
• Interleukin-6 (genetics, immunology)
• Mice
• Mice, Inbred C57BL
• NF-kappa B (genetics, immunology)
• Pleurotus (chemistry)
• Polysaccharides (administration & dosage, analysis)
• Tumor Necrosis Factor-alpha (genetics, immunology)