Abstract | BACKGROUND: METHODS: Human umbilical vein endothelial cells (HUVEC) were stimulated with 100 μmol/L palmitate (PA) for 30 min and the effects of 30 min pretreatment with 0.1-10 μmol/L kakkalide on reactive oxygen species (ROS)-associated inflammation in cells were evaluated by western blot analysis and reverse transcription-polymerase chain reaction. Furthermore, we investigated the biomodulation of insulin signaling by kakkalide along the insulin receptor substrate (IRS)-1/Akt/ endothelial nitric oxide synthase (eNOS) pathway. We also determined the effects of 30 min pretreatment with 0.1-10 μmol/L kakkalide on insulin-mediated endothelium-dependent vasodilation of rat aorta in vitro following stimulation with 100 μmol/L PA. RESULTS: CONCLUSIONS:
Kakkalide inhibited ROS-associated inflammation and ameliorated insulin-resistant endothelial dysfunction by beneficial effects on IRS-1 function.
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Authors | Dongyan Zhang, Xuejiao Gao, Qi Wang, Minjian Qin, Kang Liu, Fang Huang, Baolin Liu |
Journal | Journal of diabetes
(J Diabetes)
Vol. 5
Issue 1
Pg. 13-24
(Mar 2013)
ISSN: 1753-0407 [Electronic] Australia |
PMID | 23190749
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | © 2012 Ruijin Hospital, Shanghai Jiaotong University School of Medicine and Wiley Publishing Asia Pty Ltd. |
Chemical References |
- DNA Primers
- Glycosides
- IRS1 protein, human
- Insulin
- Insulin Receptor Substrate Proteins
- Isoflavones
- Reactive Oxygen Species
- Nitric Oxide
- kakkalide
- Phosphatidylinositol 3-Kinases
- Proto-Oncogene Proteins c-akt
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Topics |
- Base Sequence
- Blotting, Western
- Cells, Cultured
- DNA Primers
- Endothelium, Vascular
(cytology, drug effects, metabolism)
- Glycosides
(pharmacology)
- Humans
- Insulin
(metabolism, physiology)
- Insulin Receptor Substrate Proteins
(metabolism)
- Insulin Resistance
- Isoflavones
(pharmacology)
- Membrane Potential, Mitochondrial
(drug effects)
- Nitric Oxide
(biosynthesis)
- Phosphatidylinositol 3-Kinases
(metabolism)
- Phosphorylation
- Proto-Oncogene Proteins c-akt
(metabolism)
- Reactive Oxygen Species
(metabolism)
- Reverse Transcriptase Polymerase Chain Reaction
- Signal Transduction
- Vasodilation
(drug effects, physiology)
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