Resveratrol mitigates rat retinal ischemic injury: the roles of matrix metalloproteinase-9, inducible nitric oxide, and heme oxygenase-1.

Abstract

PURPOSE:

Retinal ischemia-associated ocular disorders, such as retinal occlusive disorders, neovascular age-related macular degeneration, proliferative diabetic retinopathy, and glaucoma are vision-threatening. In this study, we examined whether and by what mechanisms resveratrol, a polyphenol found in red wine, is able to protect against retinal ischemia/reperfusion injury.

METHODS:

In vivo rat retinal ischemia was induced by high intraocular pressure (HIOP), namely, 120 mmHg for 60 min. The mechanism and management was evaluated by electroretinogram (ERG) b-wave amplitudes measurement, immunohistochemistry, and real-time polymerase chain reaction.

RESULTS:

The HIOP-induced retinal ischemic changes were characterized by a decrease in ERG b-wave amplitudes, a loss of choline acetyltransferase immunolabeling of amacrine cell bodies/neuronal processes, and increased vimentin immunoreactivity, which is a marker of Müller cells, together with upregulation of matrix metalloproteinase-9 (MMP-9), heme oxygenase-1 (HO-1), and inducible nitric oxide (iNOS), and downregulation of Thy-1, both at the mRNA level. The detrimental effects due to the ischemia were concentration-dependent (weaker effect at 0.05 nmole) and/or significantly (at 0.5 nmole) altered when resveratrol was applied 15 min before or after retina ischemia.

CONCLUSION:

This study supports the hypothesis that resveratrol may be able to protect the retina against ischemia by downregulation of MMP-9 and iNOS, and upregulation of HO-1.

• Authors: Xiao-Qian Liu, Bing-Jhih Wu, Wynn H T Pan, Xiu-Mei Zhang, Jorn-Hon Liu, Mi-Mi Chen, Fang-Ping Chao, Hsiao-Ming Chao
• Journal: Journal of ocular pharmacology and therapeutics : the official journal of the Association for Ocular Pharmacology and Therapeutics (J Ocul Pharmacol Ther) Vol. 29 Issue 1 Pg. 33-40 (Feb 2013) ISSN: 1557-7732 [Electronic] United States
• PMID: 23075401 (Publication Type: Journal Article)

Chemical References

• RNA, Messenger
• Stilbenes
• Thy-1 Antigens
• Vimentin
• Nitric Oxide Synthase Type II
• Heme Oxygenase-1
• Matrix Metalloproteinase 9
• Resveratrol

Topics

• Animals
• Dose-Response Relationship, Drug
• Down-Regulation (drug effects)
• Electroretinography
• Heme Oxygenase-1 (genetics)
• Intraocular Pressure
• Matrix Metalloproteinase 9 (genetics)
• Nitric Oxide Synthase Type II (genetics)
• RNA, Messenger (metabolism)
• Rats
• Rats, Wistar
• Real-Time Polymerase Chain Reaction
• Reperfusion Injury (drug therapy, pathology)
• Resveratrol
• Retina (drug effects, pathology)
• Retinal Vessels (drug effects, pathology)
• Stilbenes (administration & dosage, pharmacology)
• Thy-1 Antigens (genetics)
• Up-Regulation (drug effects)
• Vimentin (immunology)