Abstract | BACKGROUND: RESULTS: In wild type (WT) mice, the subplantar administration of morphine dose-dependently decreased the mechanical and thermal allodynia induced by the chronic constriction of the sciatic nerve (CCI), which effects were significantly diminished after their co-administration with different subanalgesic doses of a selective NOS1 (N-[(4S)-4-amino-5-[(2-aminoethyl)amino]pentyl]-N'- nitroguanidine tris( trifluoroacetate) salt; NANT), NOS2 (L-N(6)-(1-iminoethyl)- lysine; L-NIL), L- guanylate cyclase (1H-[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one; ODQ), PKG ((Rp)-8-(para-chlorophenylthio) guanosine-3',5'-cyclic monophosphorothioate; Rp-8-pCPT-cGMPs) inhibitor or a KATP channel blocker ( glibenclamide). The evaluation of the expression of MOR in the dorsal root ganglia from sham-operated and sciatic nerve-injured WT, NOS1 knockout (KO) and NOS2-KO mice at 21 days after surgery demonstrated that, although the basal mRNA and protein levels of MOR were similar between WT and both NOS-KO animals, nerve injury only decreased their expression in WT mice. CONCLUSIONS: These results suggest that the peripheral nitric oxide-cGMP-PKG-KATP signaling pathway activation participates in the local antiallodynic effects of morphine after sciatic nerve injury and that nitric oxide, synthesized by NOS1 and NOS2, is implicated in the dorsal root ganglia down-regulation of MOR during neuropathic pain.
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Authors | Arnau Hervera, Roger Negrete, Sergi Leánez, Jesús M Martín-Campos, Olga Pol |
Journal | Molecular pain
(Mol Pain)
Vol. 7
Pg. 25
(Apr 12 2011)
ISSN: 1744-8069 [Electronic] United States |
PMID | 21486477
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- CTAP octapeptide
- Peptide Fragments
- Quaternary Ammonium Compounds
- Receptors, Opioid, mu
- Nitric Oxide
- Naloxone
- Somatostatin
- N-methylnaloxone
- Morphine
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Topics |
- Animals
- Ganglia, Spinal
(drug effects, metabolism)
- Hyperalgesia
(drug therapy, etiology)
- Mice
- Mice, Knockout
- Morphine
(antagonists & inhibitors, pharmacology, therapeutic use)
- Naloxone
(analogs & derivatives, pharmacology)
- Neuralgia
(drug therapy, metabolism)
- Nitric Oxide
(metabolism)
- Peptide Fragments
(pharmacology)
- Quaternary Ammonium Compounds
(pharmacology)
- Receptors, Opioid, mu
(antagonists & inhibitors, genetics, metabolism)
- Sciatic Nerve
(injuries)
- Signal Transduction
(drug effects)
- Somatostatin
(pharmacology)
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