Abstract | AIMS/HYPOTHESIS:
Insulin has anti-inflammatory effects in short-term experiments. However, the effects of chronic insulin administration on inflammation are unknown. We hypothesised that chronic insulin administration would beneficially alter adipose tissue inflammation and several circulating inflammatory markers. METHODS: We administered two forms of long-acting insulin, insulin glargine (A21Gly,B31Arg,B32Arg human insulin) and insulin detemir (B29Lys[ε-tetradecanoyl],desB30 human insulin), to LDL-receptor-deficient mice. After 8 weeks on a diet that causes obesity, hyperglycaemia, adipose tissue macrophage accumulation and atherosclerosis, the mice received subcutaneous glargine, detemir or NaCl (control) for 12 weeks. Serum amyloid A (SAA) and serum amyloid P (SAP), metabolic variables, adipose tissue macrophages and aortic atherosclerosis were evaluated. RESULTS: CONCLUSIONS/INTERPRETATION: While chronic insulin administration did not decrease SAA and SAP, administration of glargine but not detemir insulin improved dyslipidaemia, IL-6 levels and atherosclerosis, and both insulins reduced macrophage accumulation in visceral adipose tissue. Thus, chronic insulin therapy has beneficial tissue effects independent of circulating inflammatory markers in this murine model of diet-induced obesity and diabetes.
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Authors | J Yoon, S Subramanian, Y Ding, S Wang, L Goodspeed, B Sullivan, J Kim, K D O'Brien, A Chait |
Journal | Diabetologia
(Diabetologia)
Vol. 54
Issue 5
Pg. 1252-60
(May 2011)
ISSN: 1432-0428 [Electronic] Germany |
PMID | 21327868
(Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
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Chemical References |
- Insulin
- Insulin, Long-Acting
- Receptors, LDL
- Insulin Glargine
- Insulin Detemir
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Topics |
- Adipose Tissue
(cytology, drug effects)
- Animals
- Atherosclerosis
(drug therapy)
- Body Composition
(drug effects)
- Immunohistochemistry
- Insulin
(analogs & derivatives, therapeutic use)
- Insulin Detemir
- Insulin Glargine
- Insulin, Long-Acting
- Macrophages
(cytology, drug effects)
- Male
- Mice
- Mice, Knockout
- Obesity
(drug therapy)
- Polymerase Chain Reaction
- Receptors, LDL
(deficiency, genetics)
- Weight Gain
(drug effects)
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