Dual role of B cells with accelerated onset but reduced disease activity in P0₁₀₆₋₁₂₅-induced experimental autoimmune neuritis of IgH ⁰(/)⁰ mice.

Abstract

The role of B cells in autoimmune-mediated diseases of the peripheral nervous system was studied in experimental autoimmune neuritis (EAN) in B cell deficient IgH⁰(/)⁰ C57BL/6J mice having been immunized with P0₁₀₆₋₁₂₅ peptide. Compared to coisogenic IgH(+/+) mice, onset of EAN was accelerated [100% disease incidence at day 9 post immunization (p.i.) vs. day 15 p.i.]. At day 9 p.i., numbers of P0₁₀₆₋₁₂₅-specific interferon (IFN)-γ-producing CD4(+) T cells were increased, while IL-10 mRNA and production were decreased in IgH⁰(/)⁰ mice. Beyond day 9 p.i., declining disease activity and a significant reduction of maximal disease activity were correlated with significantly reduced numbers of IFN-γ-producing CD4(+) T cells in IgH(0/0) mice as compared with IgH(+/+) mice. Correspondingly, neuropathology demonstrated only mild axonal damage, while demyelination and dying back axonopathy with spinal cord motor neuron apoptosis were absent. Thus, depending on the stage of EAN, B cells play a dual, i.e. suppressive and enhancing, role during induction and at height of EAN, respectively. The combined interaction of B cells as well as CD4(+) and CD8(+) T cells is required for the development of EAN.

• Authors: Anna Brunn, Olaf Utermöhlen, Monica Sánchez-Ruiz, Manuel Montesinos-Rongen, Tobias Blau, Dirk Schlüter, Martina Deckert
• Journal: Acta neuropathologica (Acta Neuropathol) Vol. 120 Issue 5 Pg. 667-81 (Nov 2010) ISSN: 1432-0533 [Electronic] Germany
• PMID: 20640902 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Immunoglobulin G
• Immunoglobulin M
• Myelin P0 Protein
• Interleukin-10
• Interferon-gamma

Topics

• Animals
• B-Lymphocytes (immunology)
• CD4-Positive T-Lymphocytes (immunology)
• CD8-Positive T-Lymphocytes (immunology)
• Cell Separation
• Enzyme-Linked Immunosorbent Assay
• Flow Cytometry
• Genes, Immunoglobulin Heavy Chain
• Immunoglobulin G (blood)
• Immunoglobulin M (blood)
• Immunohistochemistry
• Interferon-gamma (biosynthesis, immunology)
• Interleukin-10 (biosynthesis, immunology)
• Mice
• Mice, Inbred C57BL
• Mice, Knockout
• Myelin P0 Protein (immunology)
• Neuritis, Autoimmune, Experimental (genetics, immunology, pathology)
• Reverse Transcriptase Polymerase Chain Reaction
• Sciatic Nerve (immunology, pathology)