[Role of leptin in hepatic ischemia/reperfusion-induced hepatic injury].

Abstract

UNLABELLED:

OBJECTIVE; To study the changes of leptin after hepatic ischemia/reperfusion (H-I/R) and its effects on H-I/R-induced hepatic injury.

METHODS:

A 70% H-I/R model of rats was established. The rats were divided into groups with different reperfusion times and sham-operation group. Radioimmunoassay was applied to measure protein levels of leptin in serum and adipose tissues of the rats. Enzyme-colorimetry was used to detect serum alanine transaminase. Hematoxylin-eosin staining and immunohistochemistry were applied to investigate pathological variations and protein expressions of leptin in livers, respectively. RT-PCR was used to detect leptin mRNA expressions in adipose tissues and livers.

RESULTS:

Compared with the sham-operation group, serum leptin increased significantly in the 60 min ischemia/360 min reperfusion (I60' R360') group; protein level of leptin in adipose tissues increased significantly in the I60'R60' group; serum alanine transaminase increased significantly in all of the four reperfusion groups; protein expressions of leptin in livers increased significantly in the I60'R60' and 160'R240' groups; leptin mRNA expression in adipose tissues decreased significantly in the I60'R150' group; leptin mRNA expression in livers increased significantly in the 160'R60' group; leptin mRNA expressions in livers decreased significantly in the I60'R150', I60'R240' and I60'R360' groups. Pathological investigation showed that hepatic impairments at the early phase of H-I/R were more serious. The impairments at the later phase lessened gradually.

CONCLUSION:

The change of leptin expressions after H-I/R may be a protective factor to withstand H-I/ R-induced hepatic injury.

• Authors: Ji Lin, Guang-Tao Yan, Hui Xue, Xiu-Hua Hao, Kai Zhang, Lu-Huan Wang
• Journal: Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition (Sichuan Da Xue Xue Bao Yi Xue Ban) Vol. 40 Issue 6 Pg. 1003-7, 1043 (Nov 2009) ISSN: 1672-173X [Print] China
• PMID: 20067107 (Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Leptin
• RNA, Messenger

Topics

• Animals
• Leptin (genetics, metabolism)
• Liver (blood supply, metabolism, pathology)
• Male
• RNA, Messenger (genetics, metabolism)
• Random Allocation
• Rats
• Rats, Sprague-Dawley
• Reperfusion Injury (metabolism)
• Reverse Transcriptase Polymerase Chain Reaction