Peptidic sequence "HSEAETGPP" is recognized by the sera of pars planitis patients.

Abstract

PURPOSE:

HLA class II, p-36 protein, heat shock protein and retinal antigens have been associated with pars planitis (PP), but their participation in the development of the disease are unknown. A search for new molecules related to PP is necessary. This work focused on the identification of peptides recognized by PP patient sera using the phage display method.

METHODS:

Sera of PP patients were used to isolate peptides fused to M13-phage pIII protein. The response of PP and healthy sera to peptides was determined by ELISA. PCR amplification and sequencing of peptide-encoding fragments from clones with high recognition by PP sera were used to characterize displayed peptides.

RESULTS:

One hundred clones were randomly selected from a phage display library after three panning rounds using serum proteins from a PP patient. The immunologic response level of 100 clones selected were determined with a major number of patients, it was found that one clone was recognized stronger in PP patients sera than in healthy sera (PP vs. healthy; P < 0.05). The peptide-encoding region of this clone was sequenced and translated. The peptide sequence corresponded to HSEAETGPP. An identical amino acid sequence to HSEAETGPP is found in the human proline-rich transmembrane protein 2 which has not been related with eye diseases.

CONCLUSION:

These results suggest that the peptide HSEAETGPP is associated with PP.

• Authors: Jorge I Castañeda-Sánchez, Everardo Curiel-Quesada, Miguel Pedrosa-Seres, Mario E Cancino-Diaz, Sandra Rodríguez-Martínez, Juan C Cancino-Diaz
• Journal: Clinical and investigative medicine. Medecine clinique et experimentale (Clin Invest Med) Vol. 32 Issue 3 Pg. E206-11 (Jun 01 2009) ISSN: 1488-2353 [Electronic] Canada
• PMID: 19480736 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Peptide Library
• Peptides

Topics

• Amino Acid Sequence
• Enzyme-Linked Immunosorbent Assay
• Humans
• Pars Planitis (blood, immunology)
• Peptide Library
• Peptides (chemistry, genetics, immunology)
• Polymerase Chain Reaction