SIRT1 regulates tyrosine hydroxylase expression and differentiation of neuroblastoma cells via FOXO3a.

Abstract	To examine the function of SIRT1 in neuronal differentiation, we employed all-trans retinoic acid (ATRA)-induced differentiation of neuroblastoma cells. Nicotinamide inhibited neurite outgrowth and tyrosine hydroxylase (TH) expression. Inhibition of PARP or histone deacetylase did not inhibit TH expression, showing the effect to be SIRT1 specific. Expression of FOXO3a and its target proteins were increased during the differentiation and reduced by nicotinamide. FOXO3a deacetylation was increased by ATRA and blocked by nicotinamide. SIRT1 and FOXO3a siRNA inhibited ATRA-induced up-regulation of TH and differentiation. Taken together, these results indicate that SIRT1 is involved in ATRA-induced differentiation of neuroblastoma cells via FOXO3a.
Authors	Min-Ju Kim, Kyungsook Ahn, Seong-Hoon Park, Hong-Jun Kang, Bong Geom Jang, Soo-Jin Oh, Sun-Mee Oh, Yu-Jin Jeong, Jee-In Heo, Jun-Gyo Suh, Soon Sung Lim, Yoon-Jung Ko, Sung-Oh Huh, Sung Chan Kim, Jae-Bong Park, Jaebong Kim, Jong-Il Kim, Sangmee Ahn Jo, Jae-Yong Lee
Journal	FEBS letters (FEBS Lett) Vol. 583 Issue 7 Pg. 1183-8 (Apr 02 2009) ISSN: 1873-3468 [Electronic] England
PMID	19285077 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References	Antineoplastic Agents COL11A2 protein, human Collagen Type XI FOXO3 protein, human Forkhead Box Protein O3 Forkhead Transcription Factors Neoplasm Proteins Vitamin B Complex Niacinamide Tretinoin Tyrosine 3-Monooxygenase SIRT1 protein, human Sirtuin 1 Sirtuins Histone Deacetylases
Topics	Antineoplastic Agents (pharmacology) Cell Differentiation Cell Line, Tumor Collagen Type XI (metabolism) Forkhead Box Protein O3 Forkhead Transcription Factors (genetics, metabolism) Gene Expression Regulation, Enzymologic Gene Expression Regulation, Neoplastic Histone Deacetylases (metabolism) Humans Neoplasm Proteins (genetics, metabolism) Neuroblastoma (metabolism, pathology) Neurons (metabolism, pathology) Niacinamide (pharmacology) Sirtuin 1 Sirtuins (genetics, metabolism) Tretinoin (pharmacology) Tyrosine 3-Monooxygenase (biosynthesis, genetics) Vitamin B Complex (pharmacology)

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