Abstract | OBJECTIVE: To screen differential expression genes and proteins at transcriptome and proteome levels between human gastric cancer tissue and corresponding normal mucosa. METHODS: Fresh-frozen gastric cancers were collected from patients treated at Ruijin Hospital. A total of 22 pairs of gastric cancer tissues and the corresponding noncancerous mucosa were analyzed. Commercially available cDNA microarray with 14 592 genes/ESTs was used. Genes were considered to be up-or down-regulated when the intensity ratio Cy3/ Cy5 was > or = 2 or < or = 0.5 in over 50% samples (P<0.05). Immobilized pH gradient(IPG)-based 2-DE was applied to separate the total proteins of gastric cancer tissue and paired normal tissue. After staining and analysis by software,the differential expression proteins were identified by matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF-MS) or MALDI-TOF-TOF-MS. RESULTS: As compared with corresponding noncancerous tissue, there were totally 149 up-regulating genes/ESTs and 238 down-regulating genes/ESTs in gastric cancer, including 29 genes with 3-fold over-expression ratio and 21 genes with 5-fold under-expression. Fifteen protein spots were identified successfully, among whom there were ten over-expressed and five under-expressed proteins in gastric cancer tissue compared with normal tissue. Most of over-expressed genes and proteins were related to cell motility, cell proliferation, signal transduction, while those under-expressed genes and proteins were related to defense response, toxoid metabolism. CONCLUSION:
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Authors | Wei Li, Bing-Ya Liu, Xiao-Qing Zhang, Yan-Qing Yang, Jian-Fang Li, Kai-Ling Tang, Qing-Hua Zhang, Xue-Hua Chen, Zheng-Gang Zhu |
Journal | Zhonghua wei chang wai ke za zhi = Chinese journal of gastrointestinal surgery
(Zhonghua Wei Chang Wai Ke Za Zhi)
Vol. 12
Issue 1
Pg. 52-6
(Jan 2009)
ISSN: 1671-0274 [Print] China |
PMID | 19145505
(Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Neoplasm Proteins
- Proteome
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Topics |
- Electrophoresis, Gel, Two-Dimensional
- Expressed Sequence Tags
- Gene Expression Profiling
- Gene Expression Regulation, Neoplastic
- Genes, Neoplasm
- Genome, Human
- Humans
- Neoplasm Proteins
- Neoplasm Staging
- Oligonucleotide Array Sequence Analysis
- Proteome
(metabolism)
- Proteomics
- Stomach Neoplasms
(genetics, metabolism, pathology)
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