Potential therapy of Fc-antigen combination-encoding DNA vaccination in mouse allergic airway inflammation.

Abstract

Vaccination with allergen-encoding DNA has been proposed as having potential for allergen-specific immunotherapy. In this study, we examine the therapeutic effect of allergen-encoding DNA vaccination directly to dendritic cells (DCs) on allergen-induced allergic airway inflammation in a mouse model and explore potential mechanism. Ovalbumin (OVA)-sensitized and challenged mice were immunized with DNA vaccine and received bronchoalveolar lavage (BAL) 1 day after the last challenge, to measure BAL levels of interleukin (IL)-4, IL-5, interferon (IFN)-gamma and differential cell count. Pulmonary DCs and Spleen DCs were purified and sorted according to the expression of CD(11c) (+)CD(80) (+) and CD(11c) (+)CD(86) (+) co-stimulatory molecules. Our data demonstrated that DNA vaccine therapy with OVA-Fc-pcDNA(3.1) significantly prevented OVA-increased levels of IL-4, IL-5 and the percentage of eosinophils and OVA-decreased level of IFN-gamma. OVA-Fc-pcDNA(3.1)-treated mice had less severity of airway inflammation, and lower expression of CD(11c) (+)CD(80) (+) and CD(11c) (+)CD(86) (+) on pulmonary DCs, as compared with animals with OVA-pcDNA(3.1,) pcDNA(3.1) and OVA respectively. DNA vaccine encoding both Fc and OVA was shown to be more effective than DNA vaccine encoding OVA alone. Our data indicate that Fc-antigen combination-encoding DNA vaccination has better preventive effects on antigen-induced airway inflammation by regulating DCs, and may be a new alternative therapy for asthma.

• Authors: Y Wang, G Qian, G Wang, X Cheng, C Bai, X Wang
• Journal: Clinical and experimental immunology (Clin Exp Immunol) Vol. 154 Issue 1 Pg. 115-22 (Oct 2008) ISSN: 1365-2249 [Electronic] England
• PMID: 18727625 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Immunoglobulin Fc Fragments
• Interleukin-5
• Vaccines, DNA
• Interleukin-4
• Immunoglobulin E
• Interferon-gamma
• Ovalbumin

Topics

• Animals
• Asthma (immunology, therapy)
• Bronchoalveolar Lavage Fluid (immunology)
• Dendritic Cells (immunology)
• Eosinophils (immunology)
• Flow Cytometry
• Immunization, Passive
• Immunoglobulin E (blood)
• Immunoglobulin Fc Fragments (genetics)
• Interferon-gamma (immunology)
• Interleukin-4 (analysis)
• Interleukin-5 (analysis)
• Male
• Mice
• Mice, Inbred BALB C
• Ovalbumin (genetics)
• Vaccines, DNA (administration & dosage, immunology)