Green tea seed oil reduces weight gain in C57BL/6J mice and influences adipocyte differentiation by suppressing peroxisome proliferator-activated receptor-gamma.

Abstract

Given that tea contains a number of chemical constituents possessing medicinal and pharmacological properties, green tea seed is also believed to contain many biologically active compounds such as saponin, flavonoids, vitamins, and oil materials. However, little is known about the physiologic functions of green tea seed oil. The aim of this study is to investigate the anti-obesity effects of green tea seed oil in C57BL/6J mice and in preadipocyte 3T3L-1 cell lines. In vivo, three groups of mice were fed with a standard diet, a high-fat diet containing 30% shortening, or 30% of green tea seed oil based on a standard diet for 85 days. The levels of total cholesterol, low-density lipoprotein cholesterol, high-density lipoprotein cholesterol, triglyceride, glucose, and alanine aminotransferase in blood were analyzed at the end of the study. The mice given green tea seed oil gained less weight compared to mice given the shortening diet (p < 0.01). The plasma level of total cholesterol was decreased by a significant level of 32.4% in mice given the green tea seed oil compared to the mice given the shortening diet (p < 0.01). In addition, 3T3-L1 cells were treated for 2 days to evaluate effects of green tea seed oil on adipocyte differentiation. Green tea seed oil inhibited expression of peroxisome proliferator-activated receptor-gamma(2) and CCAAT/enhancer binding protein-alpha in adipocytes and adipose tissue from the experimental animals. These results indicate that the anti-obesity effects of green tea seed oil might be, in part, through suppression of transcription factors related to adipocyte differentiation.

• Authors: Na-Hyung Kim, Sun-Kyung Choi, Su-Jin Kim, Phil-Dong Moon, Hun-Sun Lim, In-Young Choi, Ho-Jeong Na, Hyo-Jin An, Noh-Yil Myung, Hyun-Ja Jeong, Jae-Young Um, Seung-Heon Hong, Hyung-Min Kim
• Journal: Pflugers Archiv : European journal of physiology (Pflugers Arch) Vol. 457 Issue 2 Pg. 293-302 (Nov 2008) ISSN: 0031-6768 [Print] Germany
• PMID: 18696105 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Anti-Obesity Agents
• Blood Glucose
• CCAAT-Enhancer-Binding Protein-alpha
• Dietary Fats
• PPAR gamma
• Plant Oils
• RNA, Messenger
• Triglycerides
• Cholesterol
• Alanine Transaminase

Topics

• 3T3-L1 Cells
• Adipocytes (drug effects, metabolism)
• Adipogenesis (drug effects)
• Alanine Transaminase (blood)
• Animals
• Anti-Obesity Agents (pharmacology)
• Blood Glucose (drug effects)
• CCAAT-Enhancer-Binding Protein-alpha (metabolism)
• Camellia sinensis
• Cell Proliferation (drug effects)
• Cell Survival (drug effects)
• Cholesterol (blood)
• Dietary Fats (administration & dosage)
• Disease Models, Animal
• Dose-Response Relationship, Drug
• Down-Regulation
• Eating (drug effects)
• Male
• Mice
• Mice, Inbred C57BL
• Obesity (etiology, metabolism, prevention & control)
• PPAR gamma (genetics, metabolism)
• Plant Oils (pharmacology)
• RNA, Messenger (metabolism)
• Seeds
• Time Factors
• Triglycerides (blood)
• Weight Gain (drug effects)