IL-27-mediated activation of natural killer cells and inflammation produced antitumour effects for human oesophageal carcinoma cells.

Abstract

Interleukin IL-27, composed of p28 and EBV-induced gene 3 subunits, has diverse functions in enhancing cell-mediated immunity and silencing the immunity. We examined whether forced expression of the p28-linked EBI3 gene in human oesophageal carcinoma cells (Eca109) produced antitumour effects in a T cell-defective condition. Tumour growth of Eca109 cells expressing IL-27 (Eca109/IL-27) was retarded in nude mice compared with parental and vector DNA-transduced tumours and survival of the mice inoculated with Eca109/IL-27 cells was prolonged. Expression of the tumour necrosis factor-alpha, IL-1beta and IL-6 genes was up-regulated in Eca109/IL-27 tumour specimens while the tumours remained small in size but the increased transcription was subsequently down-regulated in enlarged tumours. Spleen cells from mice-bearing Eca109/IL-27 tumours produced interferon-gamma and showed YAC-1-targeted cytotoxic activities greater than those of mice inoculated with parental or vector DNA-transducer tumours. Numbers of DX5+CD69+ natural killer cells in spleen of mice-bearing Eca109/IL-27 tumours and those of CD31+ cells within Eca109/IL-27 tumours remained the same as found in mice-bearing parental or vector DNA-transduced tumours. These data collectively suggest that the IL-27-mediated antitumour effects produced in a mature T cell-defective condition were attributable to enhanced interferon-gamma production and natural killer activities.

• Authors: L Liu, S Wang, B Shan, L Shao, A Sato, K Kawamura, Q Li, G Ma, M Tagawa
• Journal: Scandinavian journal of immunology (Scand J Immunol) Vol. 68 Issue 1 Pg. 22-9 (Jul 2008) ISSN: 1365-3083 [Electronic] England
• PMID: 18482209 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Interleukins
• MYDGF protein, human
• Interleukin-12
• Interferon-gamma

Topics

• Animals
• Cytotoxicity, Immunologic
• Esophageal Neoplasms (immunology)
• Female
• Gene Expression
• Humans
• Immunohistochemistry
• Inflammation (immunology)
• Interferon-gamma (biosynthesis)
• Interleukin-12 (biosynthesis)
• Interleukins (genetics, immunology, metabolism)
• Killer Cells, Natural (immunology, metabolism)
• Mice
• Mice, Nude
• Reverse Transcriptase Polymerase Chain Reaction
• Transduction, Genetic