[Study on the DNA immunogenicity of fusion and attachment glycoproteins of Nipah virus].
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| Abstract |
The two mammalian codon optimized genes, F and G genes of Nipah virus, were generated by assembly PCR, and inserted into mammalian expression vector pCAGGS under chicken beta-actin promoter to construct pCAGG-NiV-F and pCAGG-NiV-G. Syncytium formation was induced in BHK cells by plasmid pCAGG-NiV-F and pCAGG-NiV-G transfection, which indicate recombination proteins F and G were expressed in BHK cell and possessed good biologic activity. Six-week-old female BALB/c mice were intramuscularly primed with 100 microg pCAGG-NiV-F, pCAGG-NiV-G or pCAGG-NiV-F+ pCAGG-NiV-G respectively, and boosted with same dose after 4 weeks. The sera were collected at 3 weeks post second boost. The serum IgG against Nipah virus F and G proteins was detected by indirect ELISA using recombinant Baculovirus expressed Nipah F and G glycoproteins. The results showed that specific antibodies possessed good sensitivity and specificity. Furthermore, the G and F proteins' specific antibodies could neutralize the infectivity of VSVdeltaG* F/G (the NiV F and G envelope glycoproteins psudotyped recombinant vesicular stomatitis virus expressing green fluorescence protein). And, pCAGG-NiV-G also induced higher titer of neutralizing antibody response than pCAGG-NiV-F did. The result indicates that DAN immunization is an efficient vaccine strategy against Nipah virus.
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| Authors | Xi-Jun Wang, Jin-Ying Ge, Qing-Hua Wang, Sen Hu, Xiang-Mei Lin, Zhi-Gao Bu |
| Journal | Bing du xue bao = Chinese journal of virology (Bing Du Xue Bao) Vol. 24 Issue 1 Pg. 47-52 (Jan 2008) ISSN: 1000-8721 [Print] China |
| PMID | 18320822 (Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't) |
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